Human/Mouse RelA/NFkB p65 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC5078P
Detection of RelA/NF kappa B p65 in HeLa Human Cell Line by Flow Cytometry.
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse RelA/NFkB p65 PE-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse RelA/NF kappa B p65 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 532301
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human RelA/NF kappa B p65 isoform 1
Asn456-Ser551
Accession # Q04206
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of RelA/NF kappa B p65 in HeLa Human Cell Line by Flow Cytometry. View Larger

Detection of RelA/NF kappa B p65 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse RelA/NF kappa B p65 PE-conjugated Monoclonal Antibody (Catalog # IC5078P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: RelA/NFkB p65

RelA p65 (v-rel reticuloendotheliosis viral oncogene homolog A) is a 65 kDa member of the NF kappa B family of nuclear transcription factors. Dimers of p65 with the p50 subunit are the most common form of the NF kappa B transcription factor, but dimers with it or other family members can also occur. Upon activation, RelA p65 forms an heterotetramer and moves into the nucleus where it binds to specific DNA sequences. An alternatively spliced isoform that lacks amino acids (aa) 222‑231 (p65 delta ) does not bind DNA. Over the sequence used as an immunogen, human RelA p65 shares 96% and 98% aa identity with mouse and rat RelA p65, respectively. This portion includes one of eight potential ser/thr phosphorylation sites, two acetylation sites, and most of the Rel homology domain that interacts with I kappa B inhibitors.

 

Long Name
v-rel Reticuloendotheliosis Viral Oncogene Homolog A
Entrez Gene IDs
5970 (Human)
Alternate Names
MGC131774; nf kb p65; NFkB p65; NF-kB p65; NFKB3; NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65)); Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65; p65; p65RelA; rela p65; RelA; v-rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Product Datasheets

You must select a language.

x

Citations for Human/Mouse RelA/NFkB p65 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. CD200-CD200R immune checkpoint engagement regulates ILC2 effector function and ameliorates lung inflammation in asthma
    Authors: P Shafiei-Ja, DG Helou, BP Hurrell, E Howard, C Quach, JD Painter, L Galle-Treg, M Li, YE Loh, O Akbari
    Nature Communications, 2021;12(1):2526.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes
    Authors: L Galle-Treg, I Sankaranar, BP Hurrell, E Howard, R Lo, H Maazi, G Lewis, H Banie, AL Epstein, P Hu, VK Rehan, FD Gilliland, H Allayee, P Soroosh, AH Sharpe, O Akbari
    Nat Commun, 2019;10(1):713.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity
    Authors: BP Hurrell, L Galle-Treg, PS Jahani, E Howard, DG Helou, H Banie, P Soroosh, O Akbari
    Cell Rep, 2019;29(13):4509-4524.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Mouse RelA/NFkB p65 PE-conjugated Antibody

Average Rating: 3 (Based on 1 Review)

5 Star
0%
4 Star
0%
3 Star
100%
2 Star
0%
1 Star
0%

Have you used Human/Mouse RelA/NFkB p65 PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse RelA/NFkB p65 PE-conjugated Antibody
By Anonymous on 06/04/2019
Application: WB Sample Tested: Adipose tissue Species: Human