Human Mrc2 APC‑conjugated Antibody

R&D Systems | Catalog # FAB5770A

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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 106370
View all Mrc2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line, NS0-derived human Mrc2
Gly31-Ala1414
Accession # Q9UBG0

Specificity

Detects human Mrc2 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Mrc2 APC‑conjugated Antibody

Detection of Mrc2 in THP-1 cells by Flow Cytometry.

THP-1 cells were stained with Mouse Anti-Human Mrc2 APC‑conjugated Monoclonal Antibody (Catalog # FAB5770A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Mrc2 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: THP‑1 human acute monocytic leukemia cell line

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Mrc2

Mrc2 (C-type Mannose Receptor 2),  also know as MMR2, Endocytic Receptor 180 and uPARAP,  is a 180‑kDa type I transmembrane protein. It is one of the mannose receptor (MR) family members which share a common domain organization and have a broad range of biological functions (1). Mrc2 is an endocytic receptor that is found on migrating cells, including cancer cells, macrophages, fibroblasts and endothelial cells (2). Mature human Mrc2 is composed of 1449 amino acid (aa) that includes a 1384 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 44 aa cytoplasmic domain. The ECD shows one ricin B‑type lectin domain, one fibronectin type II domain and eight C‑type lectin domains. Within the ECD, human Mrc2 shares 91% aa identity with mouse and rat Mrc2.  Mrc2 plays an important role in extracellular matrix remodeling through interaction with its ligands, including Man, Fuc, NAcGlc, collagens and urokinase plasminogen activator receptor (uPAR) (1-3). This cell surface molecule has been reported to promote cell invasion through matrix remodeling by internalizing large fragments of collagen and routing it to the lysosome for intracellular degradation and cell chemotaxis (2). It has also been reported to interact with matrix metalloprotease-13 (MMP-13) and collagen V on the cell surface (4).

References

  1. Yuan, C. et al. (2016) Biochem. J. 473:2359.
  2. Durrel, T. et al. (2018) Nat. Commun. 9:5178.
  3. Behrendt, N. et al. (2000) J. Biol. Chem. 275:1993.
  4. Englehom, L.H. et al. (2001) Lab. Invest. 81:1403.

Long Name

Mannose Receptor, C-type 2

Alternate Names

CD280, CLEC13E, Endo180, uPARAP

Entrez Gene IDs

9902 (Human); 17534 (Mouse); 498011 (Rat)

Gene Symbol

MRC2

UniProt

Q9UBG0

Additional Mrc2 Products

Product Documents for Human Mrc2 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human Mrc2 APC‑conjugated Antibody

For research use only

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