Human MSPR/Ron Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6647F
Human MSPR/Ron Fluorescein-conjugated Antibody in Flow Cytometry
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Human MSPR/Ron Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Delects human MSP R/Ron in direct ELISAs and Western blots. In these formats, approximately 25% cross‑reactivity with recombinant mouse MSP R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MSP R/Ron
Glu25-Ser956
Accession # CAA49634
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of MSP R/Ron in Human Blood Monocytes by Flow Cytometry. View Larger

Detection of MSP R/Ron in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Goat Anti-Human MSP R/Ron Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6647F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MSPR/Ron

Macrophage stimulating protein receptor (MSP R), encoded by the human Ron and the mouse Stk, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met (the receptor for hepatocyte growth factor) and chicken Sea (1, 2). This family of receptors is synthesized as a single-chain precursor that is cleaved into a mature disulfide-linked heterodimer composed of an extracellular alpha chain and a membrane spanning beta chain with intrinsic tyrosine kinase activity. Biologically active ligands (MSP and HGF) for this family of receptors are also disulfide-linked alpha -beta heterodimers. Human MSP R cDNA encodes a 1400 amino acid (aa) residue precursor protein with a 24 aa signal peptide, a 285 aa residue alpha chain (Glu25-Arg309) and a 1091 aa residue transmembrane beta chain (Gly310-Thr1400). The extracellular domain of MSP R is comprised of an N-terminal sema domain, a PSI (plexin semaphorins integrins) domain, followed by four immunoglobulin-like folds shared by plexins and transcription factors (3). The soluble sema domain binds MSP and inhibits the MSP R-dependent signaling pathways. MSP receptor is expressed in multiple tissues including specific areas of the central and peripheral nervous systems, epithelial cells along the digestive tract, skin and lung, and in subpopulations of the mononuclear phagocyte lineage (1, 2). Although free MSP alpha or beta chains have been shown to bind MSP R, only the heterodimeric MSP can induce receptor activation and cause biological activity (4, 5). MSP R associates with other transmembrane molecules including integrins, cadherins and other cytokine receptors. Transactivation and signaling crosstalk between MSP R and its associated transmembrane receptors have been demonstrated (6-8). Human MSP R shares 72% amino acid sequence identity with mouse MSP R.

References
  1. Gaudino, G. et al. (1994) EMBO J. 13:3524.
  2. Wang, M-H. et al. (1994) Science 266:117.
  3. Angelonis, D. et al. (2004) J. Biol. Chem. 279:3726.
  4. Wang, M-H. et al. (1997) J. Biol. Chem. 272:16999.
  5. Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
  6. Danilkovitch-Miagkova, A. et al. (2000) J. Biol. Chem. 275:14783.
  7. Danilkovitch-Miagkova, A. and A. Leonard (2001) Histol. Histopathol. 16:623.
  8. Santora, M. et al. (2003) Devel. Cell 2:257.
Long Name
Macrophage Stimulating Protein Receptor
Entrez Gene IDs
4486 (Human); 19882 (Mouse)
Alternate Names
CD136 antigen; CD136; CDw136c-met-related tyrosine kinase; EC 2.7.10; EC 2.7.10.1; macrophage stimulating 1 receptor (c-met-related tyrosine kinase); MSP R; MSP receptor; MSPR; MST1R; p185-Ron; Protein-tyrosine kinase 8; PTK8 protein tyrosine kinase 8; PTK8; Ron; RONmacrophage-stimulating protein receptor; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4

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