Human MSPR/Ron PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6647P
Human MSPR/Ron PE-conjugated Antibody in Flow Cytometry
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Human MSPR/Ron PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Delects human MSP R/Ron in direct ELISAs and Western blots. In these formats, approximately 25% cross‑reactivity with recombinant mouse MSP R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MSP R/Ron
Glu25-Ser956
Accession # Q04912
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of MSP R/Ron in Human Whole Blood Monocytes by Flow Cytometry. View Larger

Detection of MSP R/Ron in Human Whole Blood Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Goat Anti-Human MSP R/Ron PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6647P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MSPR/Ron

RON (Recepteur d'Origine Nantais [Receptor originating (or discovered) in Nantes (France)]; also known as MSP-R, PTK8 and MST1R) is a 180-190 kDa member of the tyrosine kinase family, protein kinase superfamily of molecules. Human mature RON is a type I transmembrane heterodimeric glycoprotein that arises from the proteolytic cleavage of a single chain proform 1376 amino acids (aa) in length. Posttranslational processing generates a 35-40 kDa a-chain (aa 25-304) coupled to a 145-150 kDa b-chain (aa 310-1400). The chains are disulfide-linked, and the transmembrane segment appears over aa 958-978. Multiple cell types are known to express RON, and these include osteoclasts, TrkA+ dorsal root ganglia neurons, macrophages, keratinized and nonkeratinized stratified squamous epithelium, intestinal Paneth cells, renal proximal tubule and mesangial cells, monocytes, neutrophils and mammary epithelium. On the cell surface, RON serves as a receptor for MSP, a 78-80 kDa soluble molecule related to HGF. Ligand binding initiates homodimerization and receptor signaling. In addition, RON is also known to heterodimerize with multiple integral membrane proteins, either individually or as part of a complex. Proteins identified to date include b1 integrins, c-MET/HGFR, E-Cadherin, the bc-chain for IL-3, EPO-R, EGFR, IGF-IR, Plexins B1 and B3, and CD44v6. RON may also be found intracellularly, where it interacts with either the androgen receptor or EGFR in the cytoplasm, or in the nucleus, where it acts as a transcription factor coupled to HIF-1a and regulates c-Jun, Bcl-2 and c-FLIP expression. There are about a dozen alternative splice forms for RON, some soluble, while others are transmembrane, and either constitutively active or biochemically inert. They hold the potential to interact with full-length RON and impact its signaling capabilities. Reflecting the diverse nature of RON-expressing cells, RON has been found to generate multiple outcomes, including the promotion of epithelial-to-mesenchymal transitions, the maintenance of M2 macrophage phenotypes, and the initiation of keratinocyte proliferation and migration. Over aa 25-956, human and mouse RON share 76% aa sequence identity.

Long Name
Macrophage Stimulating Protein Receptor
Entrez Gene IDs
4486 (Human); 19882 (Mouse)
Alternate Names
CD136 antigen; CD136; CDw136c-met-related tyrosine kinase; EC 2.7.10; EC 2.7.10.1; macrophage stimulating 1 receptor (c-met-related tyrosine kinase); MSP R; MSP receptor; MSPR; MST1R; p185-Ron; Protein-tyrosine kinase 8; PTK8 protein tyrosine kinase 8; PTK8; Ron; RONmacrophage-stimulating protein receptor; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4

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