Human Myeloperoxidase Quantikine ELISA Kit

R&D Systems | Catalog # DMYE00

R&D Systems
Discontinued Product
DMYE00 has been discontinued. An alternative/replacement product is available: DMYE00B. View all Myeloperoxidase/MPO products.

Key Product Details

Assay Length

3.5 hours or 4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (50 µL), Human Milk (13 µL)

Sensitivity

0.618 ng/mL

Assay Range

1.56-100 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
View all Myeloperoxidase/MPO ELISA Kits »

Product Summary for Human Myeloperoxidase Quantikine ELISA Kit

The Quantikine Human Myeloperoxidase Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure human Myeloperoxidase in cell culture supernates, cell lysates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Myeloperoxidase and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Myeloperoxidase showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Myeloperoxidase.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Species

Human

Specificity

Natural and recombinant human Myeloperoxidase

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Cell Lysates, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 10.3 20.4 39.4 10.3 21.7 42.8
Standard Deviation 0.75 1.5 3.6 1.0 1.9 4.0
CV% 7.2 7.6 9.1 10.0 8.8 9.2

EDTA Plasma, Heparin Plasma, Human Milk, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 15.7 32.4 64.1 19.0 38.3 69.2
Standard Deviation 1.2 2.5 4.3 1.8 3.2 4.5
CV% 7.5 7.7 6.6 9.4 8.3 6.5

Recovery for Human Myeloperoxidase Quantikine ELISA Kit

The recovery of Myeloperoxidase spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 88-119

Linearity

To assess the linearity of the assay, samples containing high concentrations of Myeloperoxidase were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Myeloperoxidase/MPO ELISA Linearity

Scientific Data Images for Human Myeloperoxidase Quantikine ELISA Kit

Human Myeloperoxidase/MPO ELISA Cell Culture Supernate/Cell Lysate/Saliva Standard Curve

Human Myeloperoxidase/MPO ELISA Cell Culture Supernate/Cell Lysate/Saliva Standard Curve

Human Myeloperoxidase/MPO ELISA Serum/Plasma/Urine Standard Curve

Human Myeloperoxidase/MPO ELISA Serum/Plasma/Urine Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Myeloperoxidase/MPO

Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide dependent formation of hypochlorus acid (HOCl) and other reactive species. Enzymatically active MPO is a disulfide-linked tetramer that contains two heme groups and two copies each of the heavy and light chains. MPO binds Albumin, MMR, Cytokeratin 1 on vascular endothelial cells, HMW Kininogen, and Integrin CD11b/CD18 on neutrophils. These interactions promote MPO clearance, a reduction of nitric oxide and bradykinin levels, reduced vasodilation, and continued neutrophil activation. Neutrophil MPO is stored in cytoplasmic azurophilic granules. Upon cellular activation and degranulation, MPO is delivered into phagosomes where it is required for the killing of phagocytosed bacteria. Activated neutrophils also release granule contents extracellularly. Elevated plasma MPO levels have been associated with a variety of clinical conditions including systemic inflammation, eclampsia, risk of cardiovascular events, vascular endothelial dysfunction, severity of multiple sclerosis, and prospective mortality and oxidative stress during hemodialysis.

Alternate Names

MPO

Entrez Gene IDs

4353 (Human); 17523 (Mouse); 303413 (Rat)

Gene Symbol

MPO

Additional Myeloperoxidase/MPO Products

Product Documents for Human Myeloperoxidase Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Myeloperoxidase Quantikine ELISA Kit

For research use only

Citations for Human Myeloperoxidase Quantikine ELISA Kit

Customer Reviews for Human Myeloperoxidase Quantikine ELISA Kit (2)

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    Sample Tested: Blood monocytes
    Verified Customer | Posted 09/11/2017
  • Name: Anonymous
    Sample Tested: Peripheral blood neutrophils
    Verified Customer | Posted 09/07/2017

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