Human Myeloperoxidase Quantikine ELISA Kit
Human Myeloperoxidase Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Cell Lysates, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 0.994 | 3.36 | 6.68 | 0.974 | 2.99 | 6.23 |
| Standard Deviation | 0.034 | 0.085 | 0.123 | 0.078 | 0.18 | 0.38 |
| CV% | 3.4 | 2.5 | 1.8 | 8 | 6 | 6 |
Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 1.03 | 3.13 | 6.63 | 1.07 | 3.29 | 6.71 |
| Standard Deviation | 0.024 | 0.046 | 0.172 | 0.12 | 0.26 | 0.55 |
| CV% | 2.3 | 1.5 | 2.6 | 10.8 | 8 | 8.2 |
Recovery
The recovery of MPO spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 98 | 92-105 |
| Platelet-poor EDTA Plasma (n=4) | 95 | 90-101 |
| Platelet-poor Heparin Plasma (n=4) | 91 | 83-100 |
| Serum (n=4) | 100 | 88-110 |
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background: Myeloperoxidase/MPO
Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide dependent formation of hypochlorus acid (HOCl) and other reactive species. Enzymatically active MPO is a disulfide-linked tetramer that contains two heme groups and two copies each of the heavy and light chains. MPO binds Albumin, MMR, Cytokeratin 1 on vascular endothelial cells, HMW Kininogen, and Integrin CD11b/CD18 on neutrophils. These interactions promote MPO clearance, a reduction of nitric oxide and bradykinin levels, reduced vasodilation, and continued neutrophil activation. Neutrophil MPO is stored in cytoplasmic azurophilic granules. Upon cellular activation and degranulation, MPO is delivered into phagosomes where it is required for the killing of phagocytosed bacteria. Activated neutrophils also release granule contents extracellularly. Elevated plasma MPO levels have been associated with a variety of clinical conditions including systemic inflammation, eclampsia, risk of cardiovascular events, vascular endothelial dysfunction, severity of multiple sclerosis, and prospective mortality and oxidative stress during hemodialysis.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- For Serum, Plasma, & Human Milk Samples: Add 50 µL of Assay Diluent to each well.
For Cell Culture Supernate, Cell Lysate, Saliva, & Urine Samples: Add 100 µL Assay Diluent to each well. - Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well.
- For Serum, Plasma, & Human Milk Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
For Cell Culture Supernate, Cell Lysate, Saliva, & Urine Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker. - Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human Myeloperoxidase Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
16
Citations: Showing 1 - 10
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Neutrophil Dysfunction in the Airways of Children with Acute Respiratory Failure Due to Lower Respiratory Tract Viral and Bacterial Coinfections
Authors: JR Grunwell, VD Giacalone, S Stephenson, C Margaroli, BS Dobosh, MR Brown, AM Fitzpatric, R Tirouvanzi
Sci Rep, 2019;9(1):2874.
Species: Human
Sample Types: Tracheal Aspirate
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Neutrophil activation and NETosis are the major drivers of thrombosis in heparin-induced thrombocytopenia
Authors: J Perdomo, HHL Leung, Z Ahmadi, F Yan, JJH Chong, FH Passam, BH Chong
Nat Commun, 2019;10(1):1322.
Species: Human
Sample Types: Plasma
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Citrullinated histone H3 as a novel prognostic blood marker in patients with advanced cancer
Authors: C Thålin, S Lundström, C Seignez, M Daleskog, A Lundström, P Henriksson, T Helleday, M Phillipson, H Wallén, M Demers
PLoS ONE, 2018;13(1):e0191231.
Species: Human
Sample Types: Plasma
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Myeloperoxidase level and inflammatory markers and lipid and lipoprotein parameters in stable coronary artery disease
Authors: E Kimak, B Zi?ba, D Duma, J Solski
Lipids Health Dis, 2018;17(1):71.
Species: Human
Sample Types: Serum
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Cardiac magnetic resonance using late gadolinium enhancement and atrial T1 mapping predicts poor outcome in patients with atrial fibrillation after catheter ablation therapy
Authors: JA Luetkens, AC Wolpers, T Beiert, D Kuetting, D Dabir, R Homsi, H Meenderman, NA Dayé, V Knappe, M Karsdal, SH Nielsen, F Genovese, F Stöckigt, M Linhart, D Thomas, G Nickenig, HH Schild, JW Schrickel, RP Andrié
Sci Rep, 2018;8(1):13618.
Species: Human
Sample Types: Serum
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The interdependencies of viral load, the innate immune response, and clinical outcome in children presenting to the emergency department with respiratory syncytial virus-associated bronchiolitis
Authors: FA Piedra, M Mei, V Avadhanula, R Mehta, L Aideyan, RP Garofalo, PA Piedra
PLoS ONE, 2017;12(3):e0172953.
Species: Human
Sample Types: Nasal Lavage Fluid
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Paraoxonase-1 and myeloperoxidase correlate with vascular biomarkers in overweight patients with newly diagnosed untreated hyperlipidaemia
Authors: A Szentpéter, N Zsíros, VE Varga, H L?rincz, M Katkó, I Seres, P Fülöp, G Paragh, M Harangi
VASA, 2017;0(0):1-7.
Species: Human
Sample Types: Serum
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Complex regulation of neutrophil-derived MMP-9 secretion in central nervous system tuberculosis
Authors: CW Ong, PJ Pabisiak, S Brilha, P Singh, F Roncaroli, PT Elkington, JS Friedland
J Neuroinflammation, 2017;14(1):31.
Species: Human
Sample Types: Whole Cells
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Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction
Authors: S Chandran, J Watkins, A Abdul-Aziz, M Shafat, PA Calvert, KM Bowles, MD Flather, SA Rushworth, AD Ryding
J Am Heart Assoc, 2017;6(5):.
Species: Human
Sample Types: Plasma
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Combined administration of anisodamine and neostigmine rescued acute lethal crush syndrome through ?7nAChR-dependent JAK2-STAT3 signaling
Sci Rep, 2016;6(0):37709.
Species: Mouse
Sample Types: Serum
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Growth differentiation factor-15 (GDF-15) levels are associated with cardiac and renal injury in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass.
Authors: Kahli A, Guenancia C, Zeller M, Grosjean S, Stamboul K, Rochette L, Girard C, Vergely C
PLoS ONE, 2014;9(8):e105759.
Species: Human
Sample Types: Plasma
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Increased levels of leukocyte-derived MMP-9 in patients with stable angina pectoris.
Authors: Jonasson S, Lundberg A, Kalvegren H, Bergstrom I, Szymanowski A, Jonasson L
PLoS ONE, 2011;6(4):e19340.
Species: Human
Sample Types: Serum
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Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma.
Authors: Bachert C, Zhang N, Holtappels G, De Lobel L, Van Cauwenberge P, Liu S, Lin P, Bousquet J, Van Steen K
J. Allergy Clin. Immunol., 2010;126(5):962.
Species: Human
Sample Types: Tissue Homogenates
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Interleukin-8 is increased in the membrane of circulating erythrocytes in patients with acute coronary syndrome.
Authors: Tziakas DN, Chalikias GK, Tentes IK, Stakos D, Chatzikyriakou SV, Mitrousi K, Kortsaris AX, Kaski JC, Boudoulas H
Eur. Heart J., 2008;29(22):2713-22.
Species: Human
Sample Types: Plasma
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Effects of atorvastatin added to inhaled corticosteroids on lung function and sputum cell counts in atopic asthma.
Authors: Hothersall EJ, Chaudhuri R, McSharry C, Donnelly I, Lafferty J, McMahon AD, Weir CJ, Meiklejohn J, Sattar N, McInnes I, Wood S, Thomson NC
Thorax, 2008;63(12):1070-5.
Species: Human
Sample Types: Sputum
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Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
J. Allergy Clin. Immunol., 2007;120(5):1118-25.
Species: Human
Sample Types: BALF
FAQs
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Does the Human Myeloperoxidase Quantikine ELISA Kit work with sputum samples?
We have not tested sputum samples in this kit. We are aware that customers have cited the use of this kit with sputum samples. See the Citations tab. Additional sample validation by the investigator may be required.
ELISA Controls
Supplemental ELISA Products
Reviews for Human Myeloperoxidase Quantikine ELISA Kit
Average Rating: 4.5 (Based on 2 Reviews)
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