Human Myocilin APC‑conjugated Antibody

R&D Systems | Catalog # FAB34461A

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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 297823
View all Myocilin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Myocilin
Arg33-Met504
Accession # Q99972

Specificity

Detects human Myocilin in direct ELISA. 

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Myocilin APC‑conjugated Antibody

Detection of Myocilin in HEK293 cell line transfected with hMYOC vs irrelevant HEK293 transfectant cells by Flow Cytometry.

HEK293 cell line transfected with hMYOC vs irrelevant HEK293 transfectant cells were stained with Mouse Anti-Human Myocilin APC‑conjugated Monoclonal Antibody (Catalog # FAB34461A, filled histogram) or isotype control antibody, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

Applications for Human Myocilin APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: See below

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Myocilin

Myocilin (also known as TIGR) is a variably glycosylated, 65 kDa, secreted polypeptide that belongs to the family of olfactomedin-related proteins. Human Myocilin is synthesized as a 490 aa precursor that contains an 18 aa signal sequence, an 84 aa N-terminus, a 53 aa alpha ‑helical leucine zipper and a 335 aa C-terminus that contains a 260 aa OLF-domain. An alternate start site generates a signal-sequenceless 504 aa mature protein. Myocilin forms nondisulfide-linked dimers and multimers. The human 32 kDa OLF-domain shares 87% aa sequence identity with the OLF-domain in bovine, rat, and mouse.

Alternate Names

GLC1A, MYOC, TIGR

Entrez Gene IDs

4653 (Human)

Gene Symbol

MYOC

UniProt

Q99972

Additional Myocilin Products

Product Documents for Human Myocilin APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Myocilin APC‑conjugated Antibody

For research use only

Related Research Areas

Wnt Signaling Modulators

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