Human Nectin-4 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2659A
Detection of Nectin‑4 in MCF‑7 Human Cell Line by Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Nectin-4 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, does not cross-react with recombinant human Nectin‑1, -2, -3, or recombinant mouse Nectin-4.
Source
Monoclonal Mouse IgG2B Clone # 337516
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Nectin-4
Gly27-Val351
Accession # Q96NY8
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Nectin‑4 in MCF‑7 Human Cell Line by Flow Cytometry. View Larger

Detection of Nectin‑4 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line were stained with Mouse Anti-Human Nectin-4 APC-conjugated Monoclonal Antibody (Catalog # FAB2659A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Nectin-4

Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.

Long Name
Poliovirus Receptor Related 4
Entrez Gene IDs
81607 (Human); 71740 (Mouse); 498281 (Rat); 102127936 (Cynomolgus Monkey)
Alternate Names
EDSS1; Ig superfamily receptor LNIR; LNIR; LNIRnectin 4; Nectin4; Nectin-4; poliovirus receptor-related 4; poliovirus receptor-related protein 4; PRR4; PRR4DKFZp686K05193; PVRL4

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Nectin-4 APC-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human Nectin-4 APC-conjugated Antibody and earn rewards!

Have you used Human Nectin-4 APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review