Human Nectin-4 Alexa Fluor® 700-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, does not cross-react with recombinant human Nectin‑1, -2, -3, or recombinant mouse Nectin-4.
  • Source
    Monoclonal Mouse IgG2B Clone # 337516
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Nectin-4
    Accession # Q96NY8
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Nectin‑4 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human Nectin‑4 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB2659N, filled histogram) or isotype control antibody (Catalog # IC0041N, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Nectin-4
Nectin-4 is a 66-70 kDa member of the nectin family, Ig superfamily of molecules. Mature Nectin-4 is a 479 amino acid (aa) Type I transmembrane glycoprotein that contains one V-type and two C2-type Ig-like domains. Nectins mediate the Ca++-independent cell adhesion in junctional complexes and connect to the Actin cytoskeleton via Afadin. Nectins are considered to first form loose cis-homodimers which aggregate and generate homooligomer in-trans, but preferentially generates Nectin-4:Nectin-1 heterooligomers. Cells known to express Nectin-4 include keratinocytes, transitional epithelium, CD49f+ CD24+ luminal mammary epithelium, tooth stratum intermedium cells, and respiratory pseudostratified ciliated columnar epithelium. It is also found on multiple carcinoma cell types. Nectin-4 can be cleaved to generate a 43-44 kDa soluble form that apparently appears only during tumorigenesis. Nectin-4 is also known to bind the measles virus, and possibly interact, indirectly or as a heterodimer, with the prolactin receptor. Over aa 27-351, human and mouse Nectin-4 share 90% aa sequence identity.
  • Long Name:
    Poliovirus Receptor Related 4
  • Entrez Gene IDs:
    81607 (Human); 71740 (Mouse)
  • Alternate Names:
    EDSS1; Ig superfamily receptor LNIR; LNIR; LNIRnectin 4; Nectin4; nectin-4; poliovirus receptor-related 4; poliovirus receptor-related protein 4; PRR4; PRR4DKFZp686K05193; PVRL4
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control

Ctrl IC0041N
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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