Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry

Cited:

Western Blot, Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 337516
View all Nectin-4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Nectin-4
Gly27-Val351
Accession # Q96NY8

Specificity

Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, does not cross-react with recombinant human Nectin‑1, -2, -3, or recombinant mouse Nectin-4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Nectin‑4 PE‑conjugated Antibody

Detection of Nectin-4 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of Nectin-4 in MCF-7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Nectin-4 PE-conjugated Monoclonal Antibody (Catalog # FAB2659P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Nectin‑4 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: MCF-7 human breast cancer cell line

Reviewed Applications

Read 2 reviews rated 5 using FAB2659P in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Nectin-4

Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.

Long Name

Poliovirus Receptor Related 4

Alternate Names

LNIR, Nectin4, PRR4, PVRL4

Entrez Gene IDs

81607 (Human); 71740 (Mouse); 498281 (Rat); 102127936 (Cynomolgus Monkey)

Gene Symbol

NECTIN4

UniProt

Q96NY8

Additional Nectin-4 Products

Product Documents for Human Nectin‑4 PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Nectin‑4 PE‑conjugated Antibody

For research use only

Citations for Human Nectin‑4 PE‑conjugated Antibody

Customer Reviews for Human Nectin‑4 PE‑conjugated Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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Customer Images

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  • Human Nectin-4 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: SK-BR-3 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 11/02/2021
    Detection of human Nectin-4 on the SKBR3 breast cancer cell line. SKBR3 cells were treated with the human Nectin-4 PE-conjugated antibody (catalog # FAB2659P) (BLUE) or mouse IgG2b PE-conjugated isotype control antibody (RED)
    Human Nectin‑4 PE‑conjugated Antibody FAB2659P
  • Human Nectin-4 PE-conjugated Antibody
    Name: Jenna Nguyen
    Application: Affinity Purification
    Sample Tested: 4T1.2 mouse mammary carcinoma cells and A20 mouse B cell lymphoma cell line
    Species: Mouse
    Verified Customer | Posted 05/07/2019

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