Human Nectin-4 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2659P
Human Nectin-4 PE-conjugated Antibody in Data
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Human Nectin-4 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, does not cross-react with recombinant human Nectin‑1, -2, -3, or recombinant mouse Nectin-4.
Source
Monoclonal Mouse IgG2B Clone # 337516
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Nectin-4
Gly27-Val351
Accession # Q96NY8
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Nectin-4 in MCF-7 Human Cell Line by Flow Cytometry. View Larger

Detection of Nectin-4 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Nectin-4 PE-conjugated Monoclonal Antibody (Catalog # FAB2659P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Nectin-4

Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.

Long Name
Poliovirus Receptor Related 4
Entrez Gene IDs
81607 (Human); 71740 (Mouse); 498281 (Rat); 102127936 (Cynomolgus Monkey)
Alternate Names
EDSS1; Ig superfamily receptor LNIR; LNIR; LNIRnectin 4; Nectin4; Nectin-4; poliovirus receptor-related 4; poliovirus receptor-related protein 4; PRR4; PRR4DKFZp686K05193; PVRL4

Product Datasheets

Citations for Human Nectin-4 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Non-transmissible MV Vector with Segmented RNA Genome Establishes Different Types of iPSCs from Hematopoietic Cells
    Authors: T Hiramoto, M Tahara, J Liao, Y Soda, Y Miura, R Kurita, H Hamana, K Inoue, H Kohara, S Miyamoto, Y Hijikata, S Okano, Y Yamaguchi, Y Oda, K Ichiyanagi, H Toh, H Sasaki, H Kishi, A Ryo, A Muraguchi, M Takeda, K Tani
    Mol. Ther., 2020;28(1):129-141.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Probing Morbillivirus Antisera Neutralization Using Functional Chimerism between Measles Virus and Canine Distemper Virus Envelope Glycoproteins
    Authors: MA Muñoz-Alía, SJ Russell
    Viruses, 2019;11(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Measles virus infection of human keratinocytes: Possible link between measles and atopic dermatitis
    Authors: G Gourru-Les, C Mathieu, T Thevenet, V Guillaume-, JF Jégou, CG Boer, K Tomczak, LM Bloyet, C Giraud, S Grande, C Goujon, C Cornu, B Horvat
    J. Dermatol. Sci, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Attenuated, oncolytic, but not wild-type measles virus infection has pleiotropic effects on human neutrophil function.
    Authors: Zhang Y, Patel B, Dey A, Ghorani E, Rai L, Elham M, Castleton AZ, Fielding AK
    J. Immunol., 2012;188(3):1002-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Tumor cell marker PVRL4 (nectin 4) is an epithelial cell receptor for measles virus.
    Authors: Noyce RS, Bondre DG, Ha MN
    PLoS Pathog., 2011;7(8):e1002240.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human Nectin-4 PE-conjugated Antibody

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Human Nectin-4 PE-conjugated Antibody
By Anonymous on 05/07/2019
Application: Affinity Purification Sample Tested: 4T1.2 mouse mammary carcinoma cells,A20 mouse B cell lymphoma cell line Species: Mouse