Human Nectin-4 Antibody (337516)

R&D Systems | Catalog # MAB2659

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 337516
View all Nectin-4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Nectin-4
Gly27-Val351
Accession # Q96NY8

Specificity

Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, does not cross-react with recombinant human Nectin‑1, -2, -3, or recombinant mouse Nectin-4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Nectin-4 Antibody (337516)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: MCF‑7 human breast cancer cell line

Reviewed Applications

Read 1 review rated 5 using MAB2659 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nectin-4

Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.

Long Name

Poliovirus Receptor Related 4

Alternate Names

LNIR, Nectin4, PRR4, PVRL4

Entrez Gene IDs

81607 (Human); 71740 (Mouse); 498281 (Rat); 102127936 (Cynomolgus Monkey)

Gene Symbol

NECTIN4

UniProt

Q96NY8

Additional Nectin-4 Products

Product Documents for Human Nectin-4 Antibody (337516)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Nectin-4 Antibody (337516)

For research use only

Citations for Human Nectin-4 Antibody (337516)

Customer Reviews for Human Nectin-4 Antibody (337516) (1)

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  • Human Nectin-4 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: human breast cancer cell line SKBR3
    Species: Human
    Verified Customer | Posted 12/23/2020
    Detection of NECTIN4 in human breast cancer cell line. SKBR3 cells were stained with 67 nM of the Human Nectin-4 Antibody (Catalog # MAB2659) or mouse IgG2b isotype control, followed by a secondary goat a-mouse IgG Fc APC antibody. Unstained cells (Red), secondary goat a-mouse IgG Fc APC antibody alone (Blue), mouse IgG2b isotype plus secondary antibody (Orange), Human Nectin-4 Antibody (Catalog # MAB2659) plus secondary antibody (Green).
    Human Nectin-4 Antibody (337516) MAB2659

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FAQs for Human Nectin-4 Antibody (337516)

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  • Q: What is the light chain sub-type for MAB2659?

    A: The light chain is Kappa. 

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