Detection of NKCC1/SLC12A2 in HEK293 Human Cell Line Transfected with Human NKCC1/SLC12A2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human NKCC1/SLC12A2 and eGFP was stained with either (A) Mouse Anti-Human NKCC1/SLC12A2 APC‑conjugated Monoclonal Antibody (Catalog # FAB8528A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Solute Carrier, family 12 (potassium/chloride transporter), member 2 (SLC12A2) encodes NKCC1, which mediates sodium and chloride transport and reabsorption. It is a membrane protein that is important in maintaining proper ionic balance and cell volume. In contrast to the renal-specific sodium/potassium/chloride cotransporter SLC12A1, SLC12A2 is expressed in many tissues, including the basolateral membrane of secretory epithelia. It is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for SLC12A2.
Sodium/Potassium/Chloride Transporter NKCC1
Entrez Gene IDs:
6558 (Human); 20496 (Mouse); 83629 (Rat)
Basolateral Na-K-Cl symporter; BSC; BSC2; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; NKCC1MGC104233; SLC12A2; solute carrier family 12 (sodium/potassium/chloride transporters), member 2; solute carrier family 12 member 2
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