Human NKp80/KLRF1 Alexa Fluor® 350-conjugated Antibody

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FAB1900U-100UG
R&D Systems Antibodies
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Human NKp80/KLRF1 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NKp80/KLRF1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NKp30, rhNKp44, rhNKp46, or recombinant mouse NKp46 is observed.
Source
Monoclonal Rat IgG2a Clone # 239127
Immunogen
Mouse myeloma cell line NS0-derived recombinant human NKp80/KLRF1
Val66-Tyr231
Accession # Q9NZS2
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Human whole blood CD56+ natural killer cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: NKp80/KLRF1

NKp80, also known as killer cell lectin-like receptor subfamily F member 1 (KLRF1), is an 80 kDa type II transmembrane, homodimeric, activating co‑receptor expressed exclusively on the surface of NK cells. Human NKp80, a member of the C-type lectin family of proteins (1‑3), is 231 aa in length with a 38 amino acid (aa) cytoplasmic region, a 21 aa transmembrane region, and a 172 aa extracellular domain (ECD). The ECD contains the C-type lectin-like domain (aa 114‑230) and four potential sites of N-linked glycosylation. Multiple splicing variants produce four isoforms for NKp80. Isoform 1 is the standard protein. There are no murine orthologs for NKp80 (4). NKp80 functions as an inhibitory receptor based on the presence of two immunoreceptor tyrosine‑based inhibitory motifs (ITIM) in the N-terminal cytoplasmic region and the lack of charged amino acids in the transmembrane region (1). However, later studies show that NKp80 displays an activating function rather than an inhibitory one (2‑3, 5). In in vitro experiments in human NK cells, NKp80 stimulates NK cell cytotoxicity and induces calcium influx after triggering by appropriate antibodies (2, 3). One study reports that NKp80 likely functions as a co‑receptor, cooperating with NKp46 and other activating receptors in NK cell cytotoxicity (2). Activation-induced C-type lectin (AICL) is the ligand for NKp80 (3). It is a myeloid-specific receptor expressed by monocytes, macrophages, and granulocytes (3). Crosslinking of both NKp80 and AICL stimulates secretion of proinflammatory cytokines suggesting that NKp80‑AICL interaction is involved in the activating crosstalk between NK cells and myeloid cells, and thus may influence the initiation and maintenance of immune responses in humans (3).

References
  1. Roda-Navarro, P. et al. (2000) Eur. J. Immunol. 30:568.
  2. Vitale, M. et al. (2001) Eur. J. Immunol. 31:233.
  3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
  4. Yokoyama, W.M. and B.F. Plougastel (2003) Nat. Rev. Immunol. 3:304.
  5. Thomas, M. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:1656.
Entrez Gene IDs
51348 (Human)
Alternate Names
Activating coreceptor NKp80; CLEC5C; CLEC5CMGC119908; C-type lectin domain family 5 member C; killer cell lectin-like receptor F1; killer cell lectin-like receptor subfamily F member 1; killer cell lectin-like receptor subfamily F, member 1; KLRF1; Lectin-like receptor F1; MGC119907; MGC119909; ML; NKp80

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