Detection of Human OASIS/CREB3L1 by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human OASIS/CREB3L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4080) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for OASIS/CREB3L1 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
OASIS (old astrocyte specifically-induced substance) is a 57‑60 kDa member of the CREB/ATF family of transcription factors. Human OASIS is 519 amino acids (aa) in length. It is an ER transmembrane protein with a cytosolic N-terminus (aa 1‑374) and a luminal C‑terminus. The N-terminus contains one b-Zip region (aa 292‑353). Misfolded proteins in the ER induce proteolytic cleavage of OASIS. The released 66 kDa N-terminus translocates to the nucleus to activate stress response genes. There is one alternate splice form that shows a deletion of aa 112‑198. Over aa 1‑111, human OASIS shares 96% aa sequence identity with mouse OASIS.
Old Astrocyte Specifically Induced Substance
Entrez Gene IDs:
90993 (Human); 26427 (Mouse); 362165 (Rat)
BBF-2 Homolog; cAMP responsive element binding protein 3-like 1; cAMP-responsive element-binding protein 3-like protein 1; CREB3L1; OASIS; OASIScyclic AMP-responsive element-binding protein 3-like protein 1; old astrocyte specifically induced substance; Old astrocyte specifically-induced substance
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but also provides information about sample types, species, and experimental conditions.
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