Human OASIS/CREB3L1 Antibody

Catalog # Availability Size / Price Qty
AF4080
AF4080-SP
Detection of Human OASIS/CREB3L1 by Western Blot.
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Product Details
Citations (1)
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Human OASIS/CREB3L1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human OASIS/CREB3L1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human OASIS/CREB3L1
Met1-Asp111
Accession # Q96BA8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human OASIS/CREB3L1 by Western Blot. View Larger

Detection of Human OASIS/CREB3L1 by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human OASIS/CREB3L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4080) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for OASIS/CREB3L1 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OASIS/CREB3L1

OASIS (old astrocyte specifically-induced substance) is a 57‑60 kDa member of the CREB/ATF family of transcription factors. Human OASIS is 519 amino acids (aa) in length. It is an ER transmembrane protein with a cytosolic N-terminus (aa 1‑374) and a luminal C‑terminus. The N-terminus contains one b-Zip region (aa 292‑353). Misfolded proteins in the ER induce proteolytic cleavage of OASIS. The released 66 kDa N-terminus translocates to the nucleus to activate stress response genes. There is one alternate splice form that shows a deletion of aa 112‑198. Over aa 1‑111, human OASIS shares 96% aa sequence identity with mouse OASIS.

Long Name
Old Astrocyte Specifically Induced Substance
Entrez Gene IDs
90993 (Human); 26427 (Mouse); 362165 (Rat)
Alternate Names
BBF-2 Homolog; cAMP responsive element binding protein 3-like 1; cAMP-responsive element-binding protein 3-like protein 1; CREB3L1; OASIS; OASIScyclic AMP-responsive element-binding protein 3-like protein 1; old astrocyte specifically induced substance; Old astrocyte specifically-induced substance

Product Datasheets

Citation for Human OASIS/CREB3L1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Transcription Factor CREB3L1 Regulates Endoplasmic Reticulum Stress Response Genes in the Osmotically Challenged Rat Hypothalamus.
    Authors: Greenwood M, Greenwood M, Paton J, Murphy D
    PLoS ONE, 2015;10(4):e0124956.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC Not Specified

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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