Detects human Oncostatin M/OSM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
mouse Oncostatin M, recombinant human (rh) CLC, rhCNTF, rhCardiotrophin-1,
rhIL-6, rhIL-11, or rhLIF is observed.
Monoclonal Mouse IgG2A Clone # 17022
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Oncostatin M/OSM Ala26-Arg221 Accession # P13725
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of Oncostatin M/OSM in Human Dendritic Cells by Flow Cytometry.
Human dendritic cells stimulated with LPS were stained with Mouse Anti-Human Oncostatin M/OSM APC‑conjugated Monoclonal Antibody (Catalog # IC2951A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Oncostatin M/OSM
Oncostatin M (OSM) is a cytokine originally isolated from medium conditioned by PMA-treated U-937 human histiocytic leukemia cells based on its ability to inhibit growth of A375 melanoma cells. The human OSM cDNA encodes a 252 amino acid pre-pro-OSM polypeptide with a 25 residue hydrophobic signal peptide and a hydrophilic C-terminal domain that are proteolytically processed to generate the 196 residue mature form of OSM. Although both mature and pro-OSM are equally active in radio-receptor assays, the mature OSM is 5- to 60-fold more active in growth inhibition assays. Thus, proteolytic processing of the pro-OSM peptide may be important in regulating the in vivo activities of OSM.
OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors. The
biological activity of human OSM is mediated either by
the LIF/OSM receptor complex composed of gp130 and LIF R alpha or by a human
OSM specific receptor composed of gp130 and OSM R alpha. The gp130, a signal transducing component ( beta subunit) of the IL-6, LIF and CNTF receptor complexes, was identified as a low-affinity OSM receptor that does not transduce OSM signals. The low affinity LIF receptor (LIF R, a gp130-related protein) has been identified to be a component of a high-affinity OSM receptor that will transduce OSM signals. Besides its growth inhibitory activities on human A375 melanoma and mouse M1 myeloid leukemic cells, as well as on other solid tumor cells, OSM also has growth stimulatory activities on normal fibroblasts, AIDS-Kaposi’s sarcoma cells, and a human erythroleukemia cell line, TF-1. Other OSM-mediated activities reported to date include: stimulation of plasminogen activator activity in cultured bovine aortic endothelial cells; regulation of IL-6 expression in human endothelial cells; and stimulation of LDL uptake and up-regulation of cell surface LDL receptors in HepG2 cells.
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