Human Otx2 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
    Detection  of Otx2 in NTera‑2 Human Cell Line by Flow Cytometry.
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Product Details
Citations (1)
Supplemental Products

Human Otx2 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Detects human Otx2 in flow cytometry.
Monoclonal Mouse IgG2B Clone # 246826
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Otx2 (isoform b)
Accession # P32243
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488


Recommended Concentration
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of Otx2 in NTera‑2 Human Cell Line by Flow Cytometry. View Larger

Detection of Otx2 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human Otx2 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC1979G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Otx2

Otx2 (orthodenticle 2) is a 289 amino acid protein that is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.

  1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95(7):3737.
Long Name
Orthodenticle Homolog 2
Entrez Gene IDs
5015 (Human)
Alternate Names
homeobox protein OTX2; MCOPS5; MGC45000; orthodenticle homeobox 2; orthodenticle homolog 2 (Drosophila); Orthodenticle homolog 2; Otx2

Product Datasheets

Citation for Human Otx2 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid
    Authors: Q Liu, K Van Bortle, Y Zhang, MT Zhao, JZ Zhang, BS Geller, JJ Gruber, C Jiang, JC Wu, MP Snyder
    Sci Rep, 2018;8(1):12960.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow


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