Human P-Selectin/CD62P Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF137
Product Details
Citations (1)
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Human P-Selectin/CD62P Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human P-Selectin in Western blots. In this format, less than 1% cross-reactivity is observed with rhE‑Selectin, rhL‑Selectin, and rmP‑Selectin.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human P-Selectin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human P-Selectin/CD62P (Catalog # ADP3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P-Selectin/CD62P

Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L Selectin,P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble P-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of P-Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

Entrez Gene IDs
6403 (Human); 20344 (Mouse)
Alternate Names
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP

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Citation for Human P-Selectin/CD62P Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Elevated platelet microparticle levels in nonvalvular atrial fibrillation: relationship to p-selectin and antithrombotic therapy.
    Authors: Choudhury A, Chung I, Blann AD, Lip GY
    Chest, 2007-03-01;131(3):809-15.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development

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