Human/Rat P-Selectin/CD62P Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human P‑Selectin/CD62P by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human P-Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF137) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for P-Selectin/ CD62P at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot View Larger

Detection of Rat P‑Selectin/CD62P by Western Blot. Western blot shows lysates of rat platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat P-Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF137) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for P-Selectin/CD62P at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human P‑Selectin/CD62P by Simple Western^TM. Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for P‑Selectin/CD62P at approximately 219 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Rat P‑Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF137) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Preparation and Storage

Background: P-Selectin/CD62P

Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L Selectin,P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble P-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of P-Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

Product Datasheets

Related Research Areas

• Adhesion Molecules in Angiogenesis
• Adhesion Molecules on Endothelial Cells
• Adhesion Molecules on VSMC
• Alpha Granule Membrane Proteins
• Alpha Granule Soluble Molecules
• Endothelial Cell Markers
• Lectins
• Platelet Activation Markers
• Platelet Adhesion Molecules
• Platelet Markers
• Regulatory T Cells (Tregs)
• Selectins