Human Patched 2/PTCH2 Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB4078X Datasheet / COA / SDS

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FAB4078X-100UG

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Human Patched 2/PTCH2 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Patched 2/PTCH2 in direct ELISA and Western Blots.

Source

Monoclonal Mouse IgG2a Clone # 403438

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Patched 2/PTCH2
Glu79-Glu392
Accession # Q9Y6C5

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Patched 2/PTCH2

Patched 2 (PTCH2) is a 180-200 kDa 12 transmembrane member of the Patched family. Human PTCH2 is 1203 amino acids (aa) in length. It has two terminal cytoplasmic domains, one between aa 1-57, and a second between aa 1115-1203. Together with Smoothened (SMO), PTCH2 forms a membrane complex that serves as a receptor for Sonic Hedgehog (SHH). Human PTCH2 likely has two isoforms. One shows an eight aa extension of the C-terminus, while a second shows a four aa substitution for the C-terminal 61 amino acids (aa 1143-1203). Over the first extracellular loop human PTCH2 shares 95% and 96% aa sequence identity with mouse and canine PTCH2, respectively.

Entrez Gene IDs

8643 (Human); 19207 (Mouse)

Alternate Names

patched (Drosophila) homolog 2; Patched 2; patched homolog 2 (Drosophila); patched homolog 2; protein patched homolog 2; ptc2; PTCH2