Human Patched 2/PTCH2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Patched 2/PTCH2 in direct ELISA and Western Blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 403438
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Patched 2/PTCH2
    Glu79-Glu392
    Accession # Q9Y6C5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human Patched 2/PTCH2 by Western Blot. Western blot shows lysates of OVCAR‑3 human ovarian carcinoma cell line, WS‑1 human fetal skin fibroblast cell line, and Bowes human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Patched 2/PTCH2 Monoclonal Antibody (Catalog # MAB4078) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Patched 2/PTCH2 at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Patched 2/PTCH2 in Human Cervical Cancer Tissue. Patched 2/PTCH2 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human Patched 2/PTCH2 Monoclonal Antibody (Catalog # MAB4078) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Patched 2/PTCH2
Patched 2 (PTCH2) is a 180-200 kDa 12 transmembrane member of the Patched family. Human PTCH2 is 1203 amino acids (aa) in length. It has two terminal cytoplasmic domains, one between aa 1-57, and a second between aa 1115-1203. Together with Smoothened (SMO), PTCH2 forms a membrane complex that serves as a receptor for Sonic Hedgehog (SHH). Human PTCH2 likely has two isoforms. One shows an eight aa extension of the C-terminus, while a second shows a four aa substitution for the C-terminal 61 amino acids (aa 1143-1203). Over the first extracellular loop human PTCH2 shares 95% and 96% aa sequence identity with mouse and canine PTCH2, respectively.
  • Entrez Gene IDs:
    8643 (Human); 19207 (Mouse)
  • Alternate Names:
    patched (Drosophila) homolog 2; Patched 2; patched homolog 2 (Drosophila); patched homolog 2; protein patched homolog 2; ptc2; PTCH2
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