Human Pax5/BSAP PE-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC3487P-025
Detection  of Pax5/BSAP in Human PBMCs by Flow Cytometry.
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Human Pax5/BSAP PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pax5/BSAP in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1207C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human Pax5/BSAP
Thr141-His391
Accession # Q02548
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of Pax5/BSAP in Human PBMCs by Flow Cytometry. View Larger

Detection of Pax5/BSAP in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Rabbit Anti-Human Pax5/BSAP PE‑conjugated Monoclonal Antibody (Catalog # IC3487P) or (B) Normal Rabbit IgG Phycoerythrin Control (Catalog # IC1051P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Pax5/BSAP

Pax5, also known as BSAP (B-cell-specific transcription factor) is a 44-48 kDa monomeric protein that belongs to the paired box transcription factor family of molecules. Human Pax5 is 391 amino acids (aa) in length and contains the paired DNA-binding domain over aa 16-142. More than 10 alternatively spliced isoforms with MW ranging from 25-40 kDa have been reported that likely possess different transactivation properties. Splicing may involve all but the first N-terminal 70 aa. Pax5 is principally found in pro-B cells and mature B cells where it promotes a B cell phenotype at the expense of plasma cell formation. In conjunction with Bcl-6, Pax5 represses XBP-1 and Blimp-1 expression. Over aa 141-391, human Pax5 shares 99% aa sequences identity with mouse Pax5.

Long Name
Paired Box Gene 5/B-cell Specific Activator Protein
Entrez Gene IDs
5079 (Human); 18507 (Mouse)
Alternate Names
B cell specific activator protein; B-cell-specific transcription factor; BSAP; BSAPB-cell lineage specific activator; paired box 5; paired box gene 5 (B-cell lineage specific activator protein); paired box gene 5 (B-cell lineage specific activator); paired box homeotic gene 5; paired box protein Pax-5; Pax5; transcription factor PAX 5

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