Human Pax5/BSAP PE‑conjugated Antibody

R&D Systems | Catalog # IC3487P

Recombinant Monoclonal Antibody.
R&D Systems
Discontinued Product
IC3487P has been discontinued. An alternative/replacement product is available: IC3487G, IC3487R. View all Pax5/BSAP products.

Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1207C
View all Pax5/BSAP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Pax5/BSAP
Thr141-His391
Accession # Q02548

Specificity

Detects human Pax5/BSAP in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Pax5/BSAP PE‑conjugated Antibody

Detection of Pax5/BSAP antibody in Human PBMCs antibody by Flow Cytometry.

Detection of Pax5/BSAP in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Rabbit Anti-Human Pax5/BSAP PE-conjugated Monoclonal Antibody (Catalog # IC3487P) or (B) Normal Rabbit IgG Phycoerythrin Control (Catalog # IC1051P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human Pax5/BSAP PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). 

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Pax5/BSAP

Pax5, also known as BSAP (B-cell-specific transcription factor) is a 44-48 kDa monomeric protein that belongs to the paired box transcription factor family of molecules. Human Pax5 is 391 amino acids (aa) in length and contains the paired DNA-binding domain over aa 16-142. More than 10 alternatively spliced isoforms with MW ranging from 25-40 kDa have been reported that likely possess different transactivation properties. Splicing may involve all but the first N-terminal 70 aa. Pax5 is principally found in pro-B cells and mature B cells where it promotes a B cell phenotype at the expense of plasma cell formation. In conjunction with Bcl-6, Pax5 represses XBP-1 and Blimp-1 expression. Over aa 141-391, human Pax5 shares 99% aa sequences identity with mouse Pax5.

Long Name

Paired Box Gene 5/B-cell Specific Activator Protein

Alternate Names

BSAP

Entrez Gene IDs

5079 (Human); 18507 (Mouse)

Gene Symbol

PAX5

UniProt

Q02548

Additional Pax5/BSAP Products

Product Documents for Human Pax5/BSAP PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human Pax5/BSAP PE‑conjugated Antibody

For research use only

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