Human Pax5/BSAP Alexa Fluor® 488-conjugated Antibody

Recombinant Monoclonal Antibody..
  • Species Reactivity
  • Specificity
    Detects human Pax5/BSAP in direct ELISAs.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1207C
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    E. coli-derived recombinant human Pax5/BSAP
    Accession # Q02548
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Pax5/BSAP in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Rabbit Anti-Human Pax5/BSAP Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC3487G) or (B) Normal Rabbit IgG Alexa Fluor 488 Control (Catalog # IC1051G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).  View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Pax5/BSAP
Pax5, also known as BSAP (B-cell-specific transcription factor) is a 44-48 kDa monomeric protein that belongs to the paired box transcription factor family of molecules. Human Pax5 is 391 amino acids (aa) in length and contains the paired DNA-binding domain over aa 16-142. More than 10 alternatively spliced isoforms with MW ranging from 25-40 kDa have been reported that likely possess different transactivation properties. Splicing may involve all but the first N-terminal 70 aa. Pax5 is principally found in pro-B cells and mature B cells where it promotes a B cell phenotype at the expense of plasma cell formation. In conjunction with Bcl-6, Pax5 represses XBP-1 and Blimp-1 expression. Over aa 141-391, human Pax5 shares 99% aa sequences identity with mouse Pax5.
  • Long Name:
    Paired Box Gene 5/B-cell Specific Activator Protein
  • Entrez Gene IDs:
    5079 (Human); 18507 (Mouse)
  • Alternate Names:
    B cell specific activator protein; B-cell-specific transcription factor; BSAP; BSAPB-cell lineage specific activator; paired box 5; paired box gene 5 (B-cell lineage specific activator protein); paired box gene 5 (B-cell lineage specific activator); paired box homeotic gene 5; paired box protein Pax-5; transcription factor PAX 5
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Alexa Fluor® 488-conjugated Control

Ctrl IC105G
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Rabbit IgG Alexa Fluor® 488-conjugated Antibody

Ctrl IC1051G
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