Human PD-1 QuicKit ELISA Summary
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
|Intra-Assay Precision||Inter-Assay Precision|
The recovery of human PD-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Media (n=4)||114||91-131|
|Cell Lysis Buffer (n=1)||117||94-133|
|EDTA Plasma (n=2)||104||91-122|
|Heparin Plasma (n=2)||85||71-103|
Human PD-1 ELISA Standard Curve
Human PD-1 QuicKit Natural Linearity Competitor Comparison Samples containing and/or spiked with high concentrations of PD-1 in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 99%-109% compared to 105%-135% for the top competitor.
Preparation and Storage
PD-1 (Programmed Death-1 receptor) is a key regulator of the threshold of immune response and peripheral immune tolerance. It is expressed on activated T cells, B cells, monocytes, and dendritic cells and binds to PD-L1 or PD-L2. PD-1 ligation induces co-inhibitory signals in T cells promoting their apoptosis, anergy, and functional exhaustion. Blockade of the PD-1: PD-L1 interaction using anti-PD-1 antibodies enhances understanding of anti-tumor immunity and advances the potential for cancer immunotherapy.
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