Human PD-1 QuicKit ELISA

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Human PD-1 ELISA Standard Curve
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Human PD-1 QuicKit ELISA Summary

Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
9.86 pg/mL
Assay Range
25.0 - 1,600 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

Product Summary

Natural and recombinant human PD-1.


Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 20 20 20 20
Mean (pg/mL) 143 871 157 990
Standard Deviation 6.17 19.4 15 57.2
CV% 4.3 2.2 9.6 5.8


The recovery of human PD-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 114 91-131
Cell Lysis Buffer (n=1) 117 94-133
EDTA Plasma (n=2) 104 91-122
Heparin Plasma (n=2) 85 71-103
Serum (n=2) 75 67-82


To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human PD-1 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. 
Human PD-1 ELISA Linearity

Scientific Data

Human PD-1 ELISA Standard Curve

Human PD-1 QuicKit Natural Linearity Competitor Comparison Samples containing and/or spiked with high concentrations of PD-1 in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 99%-109% compared to 105%-135% for the top competitor.

Product Datasheets

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Preparation and Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PD-1

PD-1 (Programmed Death-1 receptor) is a key regulator of the threshold of immune response and peripheral immune tolerance. It is expressed on activated T cells, B cells, monocytes, and dendritic cells and binds to PD-L1 or PD-L2. PD-1 ligation induces co-inhibitory signals in T cells promoting their apoptosis, anergy, and functional exhaustion. Blockade of the PD-1: PD-L1 interaction using anti-PD-1 antibodies enhances understanding of anti-tumor immunity and advances the potential for cancer immunotherapy.

Long Name:
Programmed Death-1
Entrez Gene IDs:
5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)
Alternate Names:
CD279 antigen; CD279; hPD-1; PD1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2


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