Human Phospho-53BP1 (S25) Alexa Fluor® 405-conjugated Antibody

Catalog #: AF3405V Datasheet / COA / SDS

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AF3405V-100UG

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Human Phospho-53BP1 (S25) Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human 53BP1 when phosphorylated at S25.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human 53BP1 S25 site

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: 53BP1

The p53-binding protein 1 (53BP1) localizes to sites of damaged DNA and is phosphorylated by the ATM checkpoint kinase. 53BP1 is required for phosphorylation of other downstream ATM substrates, such as p53 and SMC-1, and is itself a substrate for the ATM kinase. ATM phosphorylates 53BP1 at S25, and this phosphorylation aids in 53BP1 nucleating a DNA damage response protein complex.

Long Name

p53 Binding Protein 1

Entrez Gene IDs

7158 (Human); 27223 (Mouse); 296099 (Rat)

Alternate Names

53BP1; p202; p53-binding protein 1; p53bp1; TDRD30; TP53-Binding Protein 1; TP53BP1; tumor protein 53-binding protein, 1; tumor protein p53 binding protein 1; tumor protein p53-binding protein, 1; tumor suppressor p53-binding protein 1