Human Phospho-WNK1 (T60) Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB4720X Datasheet / COA / SDS

Recombinant Monoclonal Antibody

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FAB4720X-100UG

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All WNK1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-WNK1 (T60) Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human WNK1 when phosphorylated at T60 in Western blots.

Source

Monoclonal Rabbit IgG Clone # 1259F

Purification

Protein A or G purified

Immunogen

Phosphopeptide containing human WNK1 T60 site
Accession # Q9H4A3

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: WNK1

With No Lysine (K) 1 (WNK1) belongs to a novel family of serine/threonine protein kinases involved in regulating ion transport. The WNKs contain a cysteine instead of the usual lysine in their kinase activation domains. WNK1 mutations have been implicated in Pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder characterized by hypertension, increased renal salt absorption, and impaired potassium and hydrogen excretion. WNK1 is phosphorylated by Akt1 and Akt2 at T60, and may function as a negative regulator of insulin-stimulated mitogenesis.

Long Name

WNK Lysine-deficient Protein Kinase 1

Entrez Gene IDs

65125 (Human); 232341 (Mouse); 116477 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.1; Erythrocyte 65 kDa protein; HSAN2hereditary sensory neuropathy, type II; HSN2MGC163339; hWNK1; KDP; KDPMGC163341; KIAA0344; Kinase deficient protein; p65; PHA2C; PRKWNK1; PRKWNK1PSK; prostate-derived sterile 20-like kinase; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; protein kinase, lysine deficient 1; serine/threonine-protein kinase WNK1; WNK lysine deficient protein kinase 1; WNK1