Detects recombinant human PILR-beta in direct ELISAs and Western blots. In Western blots, approximately 10% cross reactivity with recombinant human PILR-alpha is observed and less than 5% cross-reactivity with recombinant mouse (rm) PILR-beta, rmPILR-alpha, and rmPILR-L is observed.
Polyclonal Sheep IgG
Mouse myeloma cell line NS0-derived recombinant human PILR-beta isoform 1 Gln20-Ala189 Accession # Q9UKJ0
Supplied in a saline solution containing BSA and Sodium Azide.
5 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of PILR‑ beta in Human Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Sheep Anti-Human PILR‑ beta PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB4189P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Paired immunoglobulin-like, type 2 receptor beta (PILR-beta ) is a type I transmembrane (TM) glycoprotein belonging to the Ig superfamily. It is the activating counterpart to the ITIM-bearing PILR-alpha inhibitory receptor. PILR-beta is expressed in a wide variety of hematopoietic cells, including NK cells, macrophages, dendritic cells and neutrophils. Mature human PILR-beta is a 208 amino acid (aa) protein with one V-type Ig-like extracellular domain, a truncated cytoplasmic tail, and a positively-charged residues in its TM domain that interacts with ITAM‑bearing adaptor molecules. Over aa 20-189, in their ECD, human PILR-beta and PILR-alpha share 82% aa sequence identity. The aa sequence of mouse PILR-beta ECD is only 43% identical to that of human PILR-beta ECD.
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