PLD1 (Phospholipase D1a) is a 110‑120 kDa member of the phospholipase D family of enzymes. It is expressed in endothelial cells and select tissues. Following activation and association with Type I alpha PIPkinase, PLD1 hydrolyzes the phosphodiester bond of membrane phosphatidylcholine, generating phosphatidic acid. Human PLD1 is 1074 amino acids in length). It contains one PX/phox homology domain (aa 84‑206), a pleckstrin homology domain (aa 219‑328), and two phosphodiesterase enzyme regions (aa 459‑486 and 891‑918). There are three splice variants. One shows a 10 aa substitution for aa 962‑1074 (PLD1d), a second shows an 84 aa substitution for aa 514‑1074 (PLD1c), and a third shows an Asn substitution for aa 585‑623 (PLD1b). Caspase cleavage of PLD1a at Gln545‑Ser546 generates a 62 kDa N-terminal, and a 61 kDa C-terminal fragment. Over aa 1‑140, human PLD1a shares 91% aa identity with mouse PLD1a.
Human PLD1 Alexa Fluor™ Plus 555‑conjugated Antibody
R&D Systems | Catalog # AF5615AFP555
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Species Reactivity
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Applications for Human PLD1 Alexa Fluor™ Plus 555‑conjugated Antibody
Western Blot
Formulation, Preparation, and Storage
Formulation
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Background: PLD1
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UniProt
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Product Documents for Human PLD1 Alexa Fluor™ Plus 555‑conjugated Antibody
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Product Specific Notices for Human PLD1 Alexa Fluor™ Plus 555‑conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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Protocols
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- Cellular Response to Hypoxia Protocols
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- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
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