Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 559830
View all Plexin B1 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Plexin B1
Accession # O43157

Specificity

Detects human Plexin B1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Plexin B1 PE‑conjugated Antibody

Detection of Plexin B1 antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

Detection of Plexin B1 in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human Plexin B1 PE-conjugated Monoclonal Antibody (Catalog # FAB37491P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Plexin B1 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Jurkat human acute T cell leukemia cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Plexin B1

Plexin B1 is a 300 kDa member of the B subfamily, Plexin family of Semaphorin receptors. Mature human Plexin B1 is a 2116 amino acid (aa) type I transmembrane (TM) glycoprotein that contains a 1471 aa extracellular domain (ECD) and a 612 aa cytoplasmic region. The ECD contains one Semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200 kDa alpha -chain (aa 20-1305) and a 100 kDa TM beta -chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. Plexin B1 is a receptor for Semaphorin 4D/CD100. It forms a receptor complex with neuropilins, MET, and EGF R2. Of three known splice variants, one shows a deletion of aa 689-871, a second shows a 53 aa substitution for aa 677‑2135, and a third shows a 53 aa substitution for the 53 aa between aa 677‑729. Over aa 35-150, human Plexin B1 shares 90% and 97% aa sequence identity with canine and mouse Plexin B1, respectively.

Alternate Names

PLXN5, PLXNB1, Transmembrane Protein SEP

Entrez Gene IDs

5364 (Human); 235611 (Mouse); 316009 (Rat)

Gene Symbol

PLXNB1

UniProt

O43157

Additional Plexin B1 Products

Product Documents for Human Plexin B1 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Plexin B1 PE‑conjugated Antibody

For research use only

Citations for Human Plexin B1 PE‑conjugated Antibody

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