Human Plexin B1 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB37491P
Detection of Plexin B1 in Jurkat Human Cell Line by Flow Cytometry.
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Product Details
Citations (2)
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Human Plexin B1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Plexin B1 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 559830
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Plexin B1
Accession # O43157
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Plexin B1 antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Plexin B1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human Plexin B1 PE-conjugated Monoclonal Antibody (Catalog # FAB37491P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Plexin B1

Plexin B1 is a 300 kDa member of the B subfamily, Plexin family of Semaphorin receptors. Mature human Plexin B1 is a 2116 amino acid (aa) type I transmembrane (TM) glycoprotein that contains a 1471 aa extracellular domain (ECD) and a 612 aa cytoplasmic region. The ECD contains one Semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200 kDa alpha -chain (aa 20-1305) and a 100 kDa TM beta -chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. Plexin B1 is a receptor for Semaphorin 4D/CD100. It forms a receptor complex with neuropilins, MET, and EGF R2. Of three known splice variants, one shows a deletion of aa 689-871, a second shows a 53 aa substitution for aa 677‑2135, and a third shows a 53 aa substitution for the 53 aa between aa 677‑729. Over aa 35-150, human Plexin B1 shares 90% and 97% aa sequence identity with canine and mouse Plexin B1, respectively.

Entrez Gene IDs
5364 (Human); 235611 (Mouse); 316009 (Rat)
Alternate Names
Plexin B1; PLXN5; PLXNB1; SEP; Transmembrane Protein SEP

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Citations for Human Plexin B1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Specific mesoderm subset derived from human pluripotent stem cells ameliorates microvascular pathology in type 2 diabetic mice
    Authors: CH Gil, D Chakrabort, CP Vieira, N Prasain, SL Calzi, SD Fortmann, P Hu, K Banno, M Jamal, C Huang, MS Sielski, Y Lin, X Huang, MD Dupont, JL Floyd, R Prasad, ALF Longhini, TJ McGill, HM Chung, MP Murphy, DN Kotton, ME Boulton, MC Yoder, MB Grant
    Science Advances, 2022-03-04;8(9):eabm5559.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Interferon-?-induced CD100 on na�ve CD8(+) T cells enhances antiviral responses to hepatitis C infection through CD72 signal transduction
    Authors: BJ Li, Y He, Y Zhang, YH Guo, Y Zhou, PX Zhang, W Wang, JR Zhao, JG Li, WZ Zuo, C Fan, ZS Jia
    J. Int. Med. Res, 2017-01-12;45(1):89-100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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