Human Polypeptide GalNAc Transferase 4/GALNT4 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF7528X Datasheet / COA / SDS

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AF7528X-100UG

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All Polypeptide GalNAc Transferase 4/GALNT4 Antibodies

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Human Polypeptide GalNAc Transferase 4/GALNT4 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Polypeptide GalNac Transferase 4/GALNT4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) GALNT1 and rhGALNT3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Polypeptide GalNac Transferase 4/GALNT4
Ala36-Lys578
Accession # Q8N4A0

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Polypeptide GalNAc Transferase 4/GALNT4

GALNT4 (Polypeptide N‑acetylgalactosaminyltransferase 4; also Polypeptide GalNAc transferase 4 and UDP-GalNAc:polypeptide Nacetylgalactosaminyltransferase 4) is a 66 kDa member of the GalNAC transferase subfamily, glycosyltransferase 4 family of enzymes. It is widely expressed, highly expressed in mucous cells. GALNT4 is found in the Golgi apparatus, and catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Human GALNT4 is a 578 amino acid (aa) type II transmembrane protein. It contains a twelve aa N-terminal cytoplasmic region and a 543 aa extracellular lumenal domain (aa 36-578). There is a potential 45 kDa splice form variant. Over aa 36-578, human GALNT4 shares 92% aa sequence identity with mouse GALNT4.

Long Name

UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4

Entrez Gene IDs

8693 (Human); 14426 (Mouse); 500826 (Rat)

Alternate Names

EC 2.4.1.41; FLJ93314; GALNACT4; GalNAc-T4; GALNT4; Polypeptide GalNAc transferase 4; polypeptide N-acetylgalactosaminyltransferase 4; pp-GaNTase 4; Protein-UDP acetylgalactosaminyltransferase 4; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4; UDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase 4 (GalNAc-T4)