Human PON1 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF5816T Datasheet / COA / SDS

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AF5816T-100UG

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Human PON1 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human PON1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PON1
Ala30-Leu355
Accession # P27169

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PON1

The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/arylesterase 1 (PON1, also known as serum paraoxonase) is a 355 amino acid, 43 kDa glycoprotein that is expressed in liver and is secreted into the bloodstream where it associates with high-density lipoproteins (HDL). Serum PON1 concentrations vary widely among normal individuals, in part due to differential expression of some polymorphisms. Sequence polymorphisms in this gene may be associated with coronary heart disease and a number of phenotypes related to diabetes. PON1 is primarily a lactonase (EC 3.1.8.1) that is thought to attenuate the oxidation of low-density lipoproteins (LDL). This may slow the initiation and progression of atherosclerosis. Human PON1 shares 83% and 81% aa identity with mouse and rat PON1, respectively.

Long Name

Paraoxonase 1

Entrez Gene IDs

5444 (Human); 18979 (Mouse); 84024 (Rat)

Alternate Names

A-esterase 1; Aromatic esterase 1; arylesterase B-type; EC 3.1.1.2; EC 3.1.1.81; EC 3.1.8.1; ESA; ESAesterase A; K-45; MVCD5; paraoxonase 1; PON 1; PON1; PONparaoxonase B-type; serum aryldiakylphosphatase; Serum aryldialkylphosphatase 1; serum paraoxonase/arylesterase 1