Human PPM1D Antibody

R&D Systems | Catalog # MAB10120

R&D Systems
Discontinued Product
MAB10120 has been discontinued. An alternative/replacement product is available: AF10120. View all PPM1D products.

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 582820
View all PPM1D Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PPM1D
Ser400-Cys605
Accession # O15297

Specificity

Detects human PPM1D in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human PPM1D Antibody

PPM1D antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

PPM1D in MCF-7 Human Cell Line.

PPM1D was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human PPM1D Monoclonal Antibody (Catalog # MAB10120) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human PPM1D Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PPM1D

Protein phosphatase 1D, or Wild-type p53-induced phosphatase 1 (WIP-1), is an enzyme encoded by de PPM1D gene. WIP-1 is involved in the negative regulation of p53 expression. WIP1 Phosphatase has been shown to be overexpressed or amplified in human cancers including breast and ovarian cancers, and has been described as a potential therapeutic target in Neuroblastoma; GSK 2830371 is a potent and selective allosteric inhibitor of Wip1 phosphatase (Tocris Cat. 5140).

Long Name

Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D

Alternate Names

PP2C-delta, Wip1

Entrez Gene IDs

8493 (Human)

Gene Symbol

PPM1D

UniProt

O15297

Additional PPM1D Products

Product Documents for Human PPM1D Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human PPM1D Antibody

For research use only

Related Research Areas

Epigenetics
Phosphatases and Regulators

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