Human PPM1D Antibody

Name: Human PPM1D Antibody
Brand: R&D Systems
SKU: MAB10120

Catalog #: MAB10120 Datasheet / COA / SDS

Newer Version Available: AF10120

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Discontinued Product

MAB10120 has been discontinued and is replaced by AF10120.

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Human PPM1D Antibody Summary

Species Reactivity

Human

Specificity

Detects human PPM1D in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 582820

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PPM1D
Ser400-Cys605
Accession # O15297

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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PPM1D in MCF-7 Human Cell Line. PPM1D was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human PPM1D Monoclonal Antibody (Catalog # MAB10120) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PPM1D

Protein phosphatase 1D, or Wild-type p53-induced phosphatase 1 (WIP-1), is an enzyme encoded by de PPM1D gene. WIP-1 is involved in the negative regulation of p53 expression. WIP1 Phosphatase has been shown to be overexpressed or amplified in human cancers including breast and ovarian cancers, and has been described as a potential therapeutic target in Neuroblastoma; GSK 2830371 is a potent and selective allosteric inhibitor of Wip1 phosphatase (Tocris Cat. 5140).

Long Name

Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D

Entrez Gene IDs

8493 (Human)

Alternate Names

EC 3.1.3.16; p53-induced protein phosphatase 1; PP2C-delta; PPM1D; protein phosphatase 1D magnesium-dependent, delta isoform; protein phosphatase 1D; protein phosphatase 2C delta isoform; Protein phosphatase 2C isoform delta; Protein phosphatase magnesium-dependent 1 delta; protein phosphatase, Mg2+/Mn2+ dependent, 1D; wild-type p53-induced phosphatase 1; Wip1; WIP1protein phosphatase Wip1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

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