Protein phosphatase 1D, or Wild-type p53-induced phosphatase 1 (WIP-1), is an enzyme encoded by de PPM1D gene. WIP-1 is involved in the negative regulation of p53 expression. WIP1 Phosphatase has been shown to be overexpressed or amplified in human cancers including breast and ovarian cancers, and has been described as a potential therapeutic target in Neuroblastoma; GSK 2830371 is a potent and selective allosteric inhibitor of Wip1 phosphatase (Tocris Cat. 5140).
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Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 582820
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Product Specifications
Immunogen
E. coli-derived recombinant human PPM1D
Ser400-Cys605
Accession # O15297
Ser400-Cys605
Accession # O15297
Specificity
Detects human PPM1D in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human PPM1D Antibody (582820)
PPM1D in MCF-7 Human Cell Line.
PPM1D was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human PPM1D Monoclonal Antibody (Catalog # MAB10120) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human PPM1D Antibody (582820)
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PPM1D
Long Name
Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D
Alternate Names
PP2C-delta, Wip1
Entrez Gene IDs
8493 (Human)
Gene Symbol
PPM1D
UniProt
Additional PPM1D Products
Product Documents for Human PPM1D Antibody (582820)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human PPM1D Antibody (582820)
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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