Detection of Human Pro MMP‑9 by Western Blot.
Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line stimulated with 100 ng/ml PMA for 24 hours under reducing (R) and non-reducing (NR) conditions. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human Pro MMP‑9 Monoclonal Antibody (Catalog # MAB9111) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Pro MMP‑9 was detected at approximately 85-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
metalloproteinases are a family of zinc and calcium dependent
endopeptidases with the combined ability to degrade all the components
of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad
range of substrates including gelatin, collagen types IV and V, elastin
and proteoglycan core protein. It is believed to act synergistically
with interstitial collagenase (MMP-1) in the degradation of fibrillar
collagens as it degrades their denatured gelatin forms. MMP-9 is
produced by keratinocytes, monocytes, macrophages and PMN leukocytes.
MMP-9 is present in most cases of inflammatory responses. Structurally,
MMP-9 maybe be divided into five distinct domains: a pro-domain which is
cleaved upon activation, a gelatin-binding domain consisting of three
contiguous fibronectin type II units, a catalytic domain containing the
zinc binding site, a proline-rich linker region, and a carboxyl terminal
Matrix Metalloproteinase 9
Entrez Gene IDs:
4318 (Human); 17395 (Mouse); 81687 (Rat)
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 22.214.171.124; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase
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