Human Protocadherin-19 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB8626A-025
Detection of Protocadherin-19 in SH‑SY5Y Human Cell Line by Flow Cytometry.
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Human Protocadherin-19 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Protocadherin-19 in direct ELISA and flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 921614
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human Protocadherin-19
Met1-Ser678
Accession # Q8TAB3
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Protocadherin-19 in SH‑SY5Y Human Cell Line by Flow Cytometry. View Larger

Detection of Protocadherin-19 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH‑SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human Protocadherin-19 APC‑conjugated Monoclonal Antibody (Catalog # FAB8626A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Protocadherin-19

Protocadherin 19 (PCDH19) is a member of the d2 subfamily of the non-(gene) clustered group of the PDCH (Protocadherin) family that belongs to the Cadherin superfamily of molecules. d2 subfamily members are characterized by both the absence of a Protein Phosphatase-1 alpha binding domain, and the presence of the two short amino acid motifs in their cytoplasmic domains. PDCH19 is found in the basal ganglia and hippocampus, and will undergo weak homophilic interaction. PDCH19 mutations have been associated with epilepsy and mental retardation. 

Entrez Gene IDs
57526 (Human); 279653 (Mouse)
Alternate Names
EFMR; EIEE9; PCDH19; Protocadherin19; Protocadherin-19

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