Detection of Human PSG by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PSG Pan Specific Monoclonal Antibody (Catalog # MAB6799) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PSG at approximately 72 and 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PSG in Human Placenta. PSG was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human PSG Pan Specific Monoclonal Antibody (Catalog # MAB6799) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Pregnancy-specific beta-1-glycoprotein 1 (PSG-1),
also called SP1, PSbG1 or B1G1 and designated CD66f, is a 54-72 kDa,
419 amino acid (aa) secreted glycoprotein of the human PSG family within the
CEA (carcinoembryonic antigen) superfamily. PSG-1 shares limited aa sequence
identity with mouse PSGs, but 84-91% aa sequence identity with human PSG-3, -4,
-6, -7, and -8. Potential isoforms of 417-428 aa vary at the extreme
C-terminus. PSG-1 is produced by syncytiotrophoblast cells and its
concentration increases in maternal plasma as pregnancy progresses. Low plasma
PSG-1 in the first or early second trimester has been correlated with fetal
growth restriction. Expression of PSGs has also been detected in choriocarcinomas,
hydatiform moles, ovarian adenocarcinomas, and breast tumors. During pregnancy,
PSG-1 may play role in placental vascular morphogenesis and creation of an
anti-inflammatory uterine environment.
Pregnancy-specific beta-1-glycoprotein Pan Specific
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