Human PTPN13/PTPL1 Antibody

R&D Systems | Catalog # MAB3577

R&D Systems
Discontinued Product
MAB3577 has been discontinued. An alternative/replacement product is available: AF3577. View all PTPN13/PTPL1 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 359313
View all PTPN13/PTPL1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PTPN13/PTPL1
His2-Arg500
Accession # Q12923

Specificity

Detects endogenous human PTPN13/PTPL1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human PTPN13/PTPL1 Antibody

Detection of Human PTPN13/PTPL1 antibody by Western Blot.

Detection of Human PTPN13/PTPL1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PTPN13/PTPL1 Monoclonal Antibody (Catalog # MAB3577) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTPN13/PTPL1 at approximately 260 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Applications for Human PTPN13/PTPL1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PTPN13/PTPL1

Protein Tyrosine Phosphatase, Non-receptor Type 13, also called PTPN13, PTPL1, FAP-1, hPTP1E, and PTP-BAS, is a 260 kDa intracellular protein that removes phosphates from tyrosine residues. Genetic aberrations in PTPN13 have been reported in a wide variety of cancers, including bone, colon, hepatocellular, and germ cell line. Elevated levels of PTPN13 in cancer cell lines have also been correlated with resistance to Fas-induced apoptosis, possibly due to prevention of CD95 reaching the cell surface.

Long Name

Protein Tyrosine Phosphatase, Non-receptor Type 13

Alternate Names

EAP-1, FAP-1, PNP1, PTP-BAS, PTP-BL, PTP1E, PTPL1, PTPLE

Entrez Gene IDs

5783 (Human); 19249 (Mouse); 498331 (Rat)

Gene Symbol

PTPN13

UniProt

Q12923

Additional PTPN13/PTPL1 Products

Product Documents for Human PTPN13/PTPL1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human PTPN13/PTPL1 Antibody

For research use only

Related Research Areas

Epigenetics
Phosphatases and Regulators

Citations for Human PTPN13/PTPL1 Antibody

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