Human PTPN13/PTPL1 Antibody

Catalog # Availability Size / Price Qty
AF3577
AF3577-SP
Detection of Human PTPN13/PTPL1 by Western Blot.
2 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human PTPN13/PTPL1 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human PTPN13/PTPL1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PTPN13/PTPL1
Met1-Arg500
Accession # Q12923
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PTPN13/PTPL1 antibody by Western Blot. View Larger

Detection of Human PTPN13/PTPL1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PTPN13/PTPL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3577) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PTPN13/PTPL1 at approximately 260 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human PTPN13/PTPL1 by Western Blot View Larger

Detection of Human PTPN13/PTPL1 by Western Blot PTPN13 regulates MDA-MB-231 cell motility and invasiveness. A: Expression of wt (N13-1, N13-2, N13-3) or catalytically inactive (CS) PTPN13 in the indicated cell clones was monitored by western blotting using anti-PTPN13 antibodies. Mock: control cells (vector alone); equal loading was verified by re-probing with an anti-actin antibody. B: Cell growth measured using the MTS assay. Results, expressed as % of Mock cells, are the mean ± s.d. of three (N13-1) four (N13-2, N13-3) or five (Mock, CS) independent experiments. C: Directional migration was assessed with the wound healing assay. C. Upper panel: Phase-contrast optical photomicrographs of the wounded area at 0 and 9h. C. Lower panel: Quantification of cell migration, expressed as % of Mock cells; mean ± s.d. of 3 (N13-1) or ≥5 (Mock, N13-2, N13-3, CS) independent experiments. **P<0.01, ****P<0.0001 versus Mock. D: Individual migration of the indicated cell clones was monitored by video microscopy and cell tracking on duplicate wells in three independent experiments. Graph represents the speed of about 1500 cells for each clone, Box-plot whiskers represent the 5 and 95 percentile values; ****P<0.0001 versus Mock and CS. E: Classification of cells (percentage) according to their migration speed (as in panel E): slow (<20µm/h), medium (20 to 40µm/h) and fast (>40µm/h). F: Cell invasiveness was evaluated with the Boyden chamber test. The percentage of cells that migrated through Matrigel-coated filters was quantified relative to the total number of seeded cells. Results, expressed as % of Mock cells, are the mean ± s.d. of five (N13-1), three (N13-2, N13-3) or two (CS) independent experiments *P<0.05 versus Mock. (C, D and F) two-tailed Student's t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31938048), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTPN13/PTPL1

Protein Tyrosine Phosphatase, Non-receptor Type 13, also called PTPN13, PTPL1, FAP-1, hPTP1E and PTP-BAS, is a 260 kDa intracellular protein that removes phosphates from tyrosine residues. Genetic aberrations in PTPN13 have been reported in a wide variety of cancers, including, bone, colon, hepatocellular, and germ cell line. Elevated levels of PTPN13 in cancer cell lines have also been correlated with resistance to Fas-induced apoptosis, possibly due to prevention of CD95 reaching the cell surface.

Long Name
Protein Tyrosine Phosphatase, Non-receptor Type 13
Entrez Gene IDs
5783 (Human); 19249 (Mouse); 498331 (Rat)
Alternate Names
EAP-1; FAP-1; hPTPE1; PNP1; protein tyrosine phosphatase, non-receptor type 13 (APO-1/CD95 (Fas)-associatedphosphatase); protein-tyrosine phosphatase 1, Fas-associated; PTP1E; PTP1EDKFZp686J1497; PTP-BAS; PTP-BASEC 3.1.3.48; PTP-BL; PTP-E1; PTPL1; PTPL1nonreceptor type 13; PTPLE; PTPN13; tyrosine-protein phosphatase non-receptor type 13

Product Datasheets

You must select a language.

x

Citations for Human PTPN13/PTPL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. PTPN13 induces cell junction stabilization and inhibits mammary tumor invasiveness
    Authors: Mohamed Hamyeh, Florence Bernex, Romain M. Larive, Aurélien Naldi, Serge Urbach, Joelle Simony-Lafontaine et al.
    Theranostics
  2. A protein phosphatase network controls the temporal and spatial dynamics of differentiation commitment in human epidermis
    Authors: Ajay Mishra, Bénédicte Oulès, Angela Oliveira Pisco, Tony Ly, Kifayathullah Liakath-Ali, Gernot Walko et al.
    eLife

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human PTPN13/PTPL1 Antibody

There are currently no reviews for this product. Be the first to review Human PTPN13/PTPL1 Antibody and earn rewards!

Have you used Human PTPN13/PTPL1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review