PPP1R9B (Protein phosphatase 1 regulatory subunit 9B; also spinophilin and neurabin-II) is a widely expressed intracellular protein that is found in both cytoplasm and nucleus. Although its predicted MW is 89 kDa, it runs anolamously at 120-140 kDa in SDS-Page. PPP1R9B is associated with actin polymerization, particularly in cells such as neurons and NK cells. In NK cells, it orchestrates actin organization around the immunological synapse, allowing for targeted release of cytotoxic granules. Human PPP1R9B is 815 amino acids (aa) in length. It contains one actin-binding region (aa 1-154), a PP1 binding domain (aa 415-492), a PZD domain (aa 493-583) and a coiled-coil region (aa 647-815). PPP1R9B is reported to form homotrimers and homotetramers. There is one isoform variant that shows an Ala insertion after both Ala158 and Ala165, coupled to a premature truncation after Arg576. Over aa 291-414, human PPP1R9B shares 91% aa identity with mouse PPP1R9B.
Human/Rat PPP1R9B Alexa Fluor™ Plus 555‑conjugated Antibody
R&D Systems | Catalog # AF6465AFP555
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Applications for Human/Rat PPP1R9B Alexa Fluor™ Plus 555‑conjugated Antibody
Immunocytochemistry
Western Blot
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Background: PPP1R9B
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Product Documents for Human/Rat PPP1R9B Alexa Fluor™ Plus 555‑conjugated Antibody
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Product Specific Notices for Human/Rat PPP1R9B Alexa Fluor™ Plus 555‑conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Protocols
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- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars