Human/Rat PPP1R9B Antibody

Catalog # Availability Size / Price Qty
AF6465
AF6465-SP
Detection of Human and Rat PPP1R9B by Western Blot.
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Product Details
Citations (1)
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Human/Rat PPP1R9B Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat PPP1R9B in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PPP1R9B
Lys289-Asp412
Accession # Q96SB3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Rat PPP1R9B antibody by Western Blot. View Larger

Detection of Human and Rat PPP1R9B by Western Blot. Western blot shows lysates of human brain tissue and rat brain tissue. PVDF Membrane was probed with 1 µg/mL of Human/Rat PPP1R9B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6465) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PPP1R9B at approximately 90-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry PPP1R9B antibody in Rat Neurons by Immunocytochemistry (ICC). View Larger

PPP1R9B in Rat Neurons. PPP1R9B was detected in immersion fixed rat hippocampal neurons (E18) using Sheep Anti-Human/Rat PPP1R9B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6465) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to synapses. Cells were co-stained using Neuron-specific beta-III Tubulin Monoclonal Antibody (Catalog # MAB1195) and the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PPP1R9B

PPP1R9B (Protein phosphatase 1 regulatory subunit 9B; also spinophilin and neurabin-II) is a widely expressed intracellular protein that is found in both cytoplasm and nucleus. Although its predicted MW is 89 kDa, it runs anolamously at 120-140 kDa in SDS-Page. PPP1R9B is associated with actin polymerization, particularly in cells such as neurons and NK cells. In NK cells, it orchestrates actin organization around the immunological synapse, allowing for targeted release of cytotoxic granules. Human PPP1R9B is 815 amino acids (aa) in length. It contains one actin-binding region (aa 1-154), a PP1 binding domain (aa 415-492), a PZD domain (aa 493-583) and a coiled-coil region (aa 647-815). PPP1R9B is reported to form homotrimers and homotetramers. There is one isoform variant that shows an Ala insertion after both Ala158 and Ala165, coupled to a premature truncation after Arg576. Over aa 291-414, human PPP1R9B shares 91% aa identity with mouse PPP1R9B.

Long Name
Protein Phosphatase 1 Regulatory Subunit 9B
Entrez Gene IDs
84687 (Human); 217124 (Mouse); 84686 (Rat)
Alternate Names
FLJ30345; neurabin II; Neurabin-2; neurabin-II; PPP1R6; PPP1R9; PPP1R9B; Protein phosphatase 1 regulatory subunit 9B; protein phosphatase 1, regulatory (inhibitor) subunit 9B; protein phosphatase 1, regulatory subunit 9B, spinophilin; SPINO; Spinophilin; Spn

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Citation for Human/Rat PPP1R9B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Ocular dominance plasticity in binocular primary visual cortex does not require C1q
    Authors: CA Welsh, CÉ Stephany, RW Sapp, B Stevens
    J. Neurosci., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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