Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all PPP1R9B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PPP1R9B
Lys289-Asp412
Accession # Q96SB3

Specificity

Detects human and rat PPP1R9B in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Rat PPP1R9B Antibody

Detection of Human and Rat PPP1R9B antibody by Western Blot.

Detection of Human and Rat PPP1R9B by Western Blot.

Western blot shows lysates of human brain tissue and rat brain tissue. PVDF Membrane was probed with 1 µg/mL of Human/Rat PPP1R9B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6465) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PPP1R9B at approximately 90-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PPP1R9B antibody in Rat Neurons by Immunocytochemistry (ICC).

PPP1R9B in Rat Neurons.

PPP1R9B was detected in immersion fixed rat hippocampal neurons (E18) using Sheep Anti-Human/Rat PPP1R9B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6465) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to synapses. Cells were co-stained using Neuron-specific beta-III Tubulin Monoclonal Antibody (Catalog # MAB1195) and the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Rat PPP1R9B Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed rat hippocampal neurons (E18)

Western Blot

1 µg/mL
Sample: Human brain tissue and rat brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PPP1R9B

PPP1R9B (Protein phosphatase 1 regulatory subunit 9B; also spinophilin and neurabin-II) is a widely expressed intracellular protein that is found in both cytoplasm and nucleus. Although its predicted MW is 89 kDa, it runs anolamously at 120-140 kDa in SDS-Page. PPP1R9B is associated with actin polymerization, particularly in cells such as neurons and NK cells. In NK cells, it orchestrates actin organization around the immunological synapse, allowing for targeted release of cytotoxic granules. Human PPP1R9B is 815 amino acids (aa) in length. It contains one actin-binding region (aa 1-154), a PP1 binding domain (aa 415-492), a PZD domain (aa 493-583) and a coiled-coil region (aa 647-815). PPP1R9B is reported to form homotrimers and homotetramers. There is one isoform variant that shows an Ala insertion after both Ala158 and Ala165, coupled to a premature truncation after Arg576. Over aa 291-414, human PPP1R9B shares 91% aa identity with mouse PPP1R9B.

Long Name

Protein Phosphatase 1 Regulatory Subunit 9B

Alternate Names

Neurabin-2, PPP1R6, SPINO, Spinophilin

Entrez Gene IDs

84687 (Human); 217124 (Mouse); 84686 (Rat)

Gene Symbol

PPP1R9B

UniProt

Q96SB3 (Human)

Additional PPP1R9B Products

Product Documents for Human/Rat PPP1R9B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Rat PPP1R9B Antibody

For research use only

Related Research Areas

Epigenetics
Phosphatases and Regulators

Citations for Human/Rat PPP1R9B Antibody

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Protocols

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