Human ROBO2 Alexa Fluor® 488-conjugated Antibody

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FAB3147G-100UG
R&D Systems Antibodies
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Human ROBO2 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ROBO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ROBO1, rhROBO3, or rhROBO4 is observed.
Source
Monoclonal Mouse IgG1 Clone # 356001
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROBO2 isoform 1
Ser22-Pro859
Accession # Q9HCK4.2
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Retinoic acid‑differentiated NTera‑2 human testicular embryonic carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ROBO2

Human ROBO2 is a 175 kDa member of the ROBO family of guidance molecules (1‑3). The term ROBO derives from round-about, a description of the circuitous pathway axons take in the absence of a functional ROBO gene (3, 4). Human ROBO2 is a type I transmembrane (TM) glycoptotein that is synthesized as a 1378 amino acid (aa) precursor. It contains a 21 aa signal sequence, an 838 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 498 aa cytoplasmic region (5, 6). The ECD contains five C2-type Ig-like domains (aa 31‑504) and three fibronectin (FN) type III domains (aa 522‑826). The cytoplasmic region contains multiple 15‑20 aa long CC (conserved cytoplasmic) motifs (C0‑C2) that are found in ROBO-1 (7, 8). Human ROBO2 has at least two potential isoforms. One isoform shows a cytoplasmic truncation of the C-terminus (aa 1186‑1378) (9). A second isoform is a 570 aa soluble form that shows a deletion of the first two and one-half C2-type Ig-like domains (aa 22‑285) and terminates after the third fibronectin domain with a unique 20 aa sequence (10). Based on mouse and rat ROBO2 GenBank sequences, considerably more alternate splice forms may occur. Human ROBO2 ECD shares 98% aa sequence identity with the ECD in mouse and canine ROBO2. ROBO2 would appear to play a number of roles in cell adhesion. In the neural tube, it is the receptor for a chemorepulsant. Axons that cross the midline are directed, presumably by ROBO2/SLIT chemorepulsion, to lateral positions in the contralateral spinal cord (6, 11, 12). In addition, ROBO2 is both permissive for neurite outgrowth (via ROBO1‑ROBO2 interaction) and inhibitory for neurite outgrowth (via ROBO2-SLIT interaction) (13, 14).

References
  1. Rajagopalan, S. et al. (2000) Neuron 28:767.
  2. Guthrie, S. (2004) Curr. Biol. 14:R632.
  3. Guthrie, S. (2001) Curr. Biol. 11:R300.
  4. Seeger, M. et al. (1993) Neuron 10:409.
  5. Kidd, T. et al. (1998) Cell 92:205.
  6. Nguyen Ba-Charvet, K.T. et al. (2001) J. Neurosci. 21:4281.
  7. Bashaw, G.J. et al. (2000) Cell 101:703.
  8. Jen, J.C. et al. (2004) Science 304:1509.
  9. GenBank Accession # Q9KCK4.
  10. Isogai, T. et al. (2002) GenBank Accession # AK074780.
  11. Mambetisaeva, E.T. et al. (2005) Dev. Dyn. 233:41.
  12. Long, H. et al. (2004) Neuron 42:213.
  13. Hivert, B. et al. (2002) Mol. Cell. Neurosci. 21:534.
  14. Lin, L. et al. (2005) Mol. Cell. Neurosci. 28:547.
Long Name
Roundabout Homolog 2
Entrez Gene IDs
6092 (Human); 268902 (Mouse); 84409 (Rat)
Alternate Names
Drosophila) homolog 2; ROBO2; roundabout, axon guidance receptor, homolog 2 (Drosophila); SAX3

Product Datasheets

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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