Human SCARA5 Alexa Fluor® 488-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human SCARA5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse SCARA5 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 673527
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human SCARA5
    Arg83-His495
    Accession # Q6ZMJ2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of SCARA5 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human SCARA5 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB4900G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SCARA5
Scavenger Receptor A5 (SCARA5), also known as Tesr, is a 70-75 kDa kDa member of the scavenger receptor "supergroup" of molecules. It is one of at least six class A members that possess a collagen-like domain and form intramembrane homotrimers (1,3). Class A scavenger receptors are type II transmembrane proteins. Mature human SCARA5 consists of a 60 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 414 aa extracellular domain (ECD) with a spacer, a collagen-like, and a Scavenger Receptor Cysteine-Rich (SRCR) domain (3-6). Within the ECD, human SCARA5 shares 87% aa sequence identity with mouse and rat SCARA5. Among other class A members, it shares 23%‑34% aa sequence identity with CL-P1, MARCO, SCARA3, and SR-A1 in the ECD. Alternate splicing generates isoforms that lack the SRCR domain, the transmembrane segment, or the 225 aa spacer region between the membrane and the collagen-like domain (6). SCARA5 is a cell surface disulfide-linked homotrimer of 200-210 kDa (5,7). It is highly expressed by a number of cell types, including testicular Sertoli cells, transitional epithelium, respiratory pseudostratified columnar epithelium, intestinal M cells, astrocytes, retinal ganglion cells, endothelial cells and likely intestinal Paneth cells (5, 8). SCARA5 binds heat-killed bacterial particles but not yeast particles or modified LDL (5). It is also recognized to bind and transport L (light)-ferritin across anatomical boundaries (8). Finally, SCARA5 has also been found in both nucleus and cytoplasm (8).
  • References:
    1. Martinez, V.G. et al. (2011) Pharmacol. Rev. 63:967.
    2. Mukhopadhyay, S. And S. Gordon (2004) Immunobiology 209:39.
    3. Zani, I.A. et al. (2015) Cells 4:178.
    4. Sarraj, M.A. et al. (2005) Dev. Dyn. 234:1026.
    5. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834.
    6. SwissProt Accession # Q6ZMJ2.
    7. Li, J.Y. et al. (2009) Dev. Cell 16:35.
    8. Mendes-Jorge, L. et al. (2014) PLoS One. 9:e106974.
  • Long Name:
    Scavenger Receptor Class A Member 5
  • Entrez Gene IDs:
    286133 (Human); 71145 (Mouse); 305974 (Rat)
  • Alternate Names:
    FLJ23907; MGC45780; SCARA5; scavenger receptor class A, member 5 (putative); Tesr; testis expressed scavenger receptor; UNQ2938/PRO28700
Related Research Areas

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC0041G 3  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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