Human SCARA5 Alexa Fluor™ Plus 405‑conjugated Antibody

R&D Systems | Catalog # AF4900AFP405

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Polyclonal Sheep IgG
View all SCARA5 Primary Antibodies »

Product Specifications

Specificity

Detects human SCARA5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse SCARA5 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human SCARA5 Alexa Fluor™ Plus 405‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SCARA5

Scavenger receptor A5 (SCARA5) is also known as testis expressed scavenger receptor (Tesr). Class A scavenger receptors, including CL-P1, MARCO, SCARA3, and SR-A1, are type II transmembrane proteins that contain a collagenous stalk (1-3). Mature human SCARA5 consists of a 60 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 414 aa extracellular domain (ECD) with a spacer, a collagen-like, and a scavenger receptor cysteine-rich (SRCR) domain (4-6). Within the ECD, human SCARA5 shares 87% aa sequence identity with mouse and rat SCARA5. It shares 23%‑34% aa sequence identity with CL-P1, MARCO, SCARA3, and SR-A1. Alternate splicing generates isoforms that lack the SRCR domain, the transmembrane segment, or the 225 aa spacer region between the membrane and the collagen-like domain (6). SCARA5 is a cell surface disulfide-linked homotrimer of > 250 kDa (5). It is highly expressed in testicular Sertoli and germ cells and more weakly in the epithelia of other tissues (4, 5). During mouse development, SCARA5 expression is attenuated in female embryos at the time of sex determination, whereas it is maintained in the developing testis (4). SCARA5 is re-expressed in the adult ovary (4). SCARA5 binds heat-killed bacterial particles but not yeast particles or modified LDL (5).

References

  1. Murphy, J.E. et al. (2005) Atherosclerosis 182:1.
  2. Mukhopadhyay, S. And S. Gordon (2004) Immunobiology 209:39.
  3. Sarrias, M.R. et al. (2004) Crit. Rev. Immunol. 24:1.
  4. Sarraj, M.A. et al. (2005) Dev. Dyn. 234:1026.
  5. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834.
  6. Accession # Q6ZMJ2.

Long Name

Scavenger Receptor Class A Member 5

Alternate Names

Tesr

Entrez Gene IDs

286133 (Human); 71145 (Mouse); 305974 (Rat)

Gene Symbol

SCARA5

UniProt

Q6ZMJ2

Additional SCARA5 Products

Product Documents for Human SCARA5 Alexa Fluor™ Plus 405‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SCARA5 Alexa Fluor™ Plus 405‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Primordial and Mature Germ Cells

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