Human Semaphorin 4D/CD100 PE-conjugated Antibody Summary
Accession # Q92854
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Semaphorin 4D/CD100 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human Semaphorin 4D/CD100 PE-conjugated Monoclonal Antibody (Catalog # FAB74701P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Semaphorin 4D/CD100
Semaphorin 4D (Sema4D)/CD100, previously Sem J, G or C-like 2, is a widely expressed 150 kDa type I transmembrane glycoprotein of the Class 4 Semaphorin family with activity in the immune and nervous systems. The 861 amino acid (aa) Semaphorin 4D contains a 710 aa extracellular domain (ECD) with a sema domain and an immunoglobulin-like domain. Within the ECD region used as an immunogen, mouse Semaphorin 4D shares 87% and 93% aa identity with human and rat Semaphorin 4D, respectively. A soluble, active 120 kDa form is produced proteolytically upon activation of B and T cells. Semaphorin 4D binds CD72 on B and dendritic cells and Plexin B1 elsewhere. In developing neurons, Semaphorin 4D assists in guidance. Semaphorin 4D is proangiogenic when expressed by tumor-associated macrophages.
Citation for Human Semaphorin 4D/CD100 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Interferon-?-induced CD100 on naï¿½ve CD8(+) T cells enhances antiviral responses to hepatitis C infection through CD72 signal transduction
Authors: BJ Li, Y He, Y Zhang, YH Guo, Y Zhou, PX Zhang, W Wang, JR Zhao, JG Li, WZ Zuo, C Fan, ZS Jia
J. Int. Med. Res, 2017;45(1):89-100.
Sample Types: Whole Cells
Applications: Flow Cytometry
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