Human Serpin F1/PEDF Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB1177S Datasheet / COA / SDS

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FAB1177S-100UG

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Human Serpin F1/PEDF Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Serpin F1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Serpin F1 is observed.

Source

Monoclonal Mouse IgG2b Clone # 187003

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Serpin F1, Mouse myeloma cell line NS0-derived recombinant human Serpin F1, and S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin F1
Asp44-Pro418
Accession # P36955

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Serpin F1/PEDF

Serpin F1, also known as pigment epithelium derived factor (PEDF), is a secreted glycoprotein that shows sequence and structural similarity with the serpin superfamily proteins, but lacks serine protease inhibitory activity. Serpin F1 has been shown to have cell-type specific functions including inhibition of retinal angiogenesis and neurotrophic activity in the retina and central nervous system.

Long Name

Serpine F1/Pigment Epithelium-derived Factor

Entrez Gene IDs

5176 (Human); 20317 (Mouse); 287526 (Rat)

Alternate Names

Cell proliferation-inducing gene 35 protein; EPC-1; EPC-1PIG35; PEDF; PEDFpigment epithelium-derived factor; pigment epithelium derived factor), member 1; proliferation-inducing protein 35; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin; Serpin F1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1