Human Serpin F1/PEDF Antibody

MAB1177 1 Citation Datasheet / COA / SDS

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MAB1177-SP
MAB1177-500
MAB1177-100

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Serpin F1/PEDF Antibody Summary

Species Reactivity

Human

Specificity

Detects human Serpin F1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Serpin F1 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 187003

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Serpin F1, Mouse myeloma cell line NS0-derived recombinant human Serpin F1, and S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin F1
Asp44-Pro418
Accession # P36955

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Serpin F1/PEDF

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human skin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin F1/PEDF

Serpin F1, also known as pigment epithelium derived factor (PEDF), is a secreted glycoprotein that shows sequence and structural similarity with the serpin superfamily proteins, but lacks serine protease inhibitory activity. Serpin F1 has been shown to have cell-type specific functions including inhibition of retinal angiogenesis and neurotrophic activity in the retina and central nervous system.

Long Name

Serpine F1/Pigment Epithelium-derived Factor

Entrez Gene IDs

5176 (Human); 20317 (Mouse); 287526 (Rat)

Alternate Names

Cell proliferation-inducing gene 35 protein; EPC-1; EPC-1PIG35; PEDF; PEDFpigment epithelium-derived factor; pigment epithelium derived factor), member 1; proliferation-inducing protein 35; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin; Serpin F1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Serine Proteases and Regulators

Citation for Human Serpin F1/PEDF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Systemically administered liposome-encapsulated Ad-PEDF potentiates the anti-cancer effects in mouse lung metastasis melanoma.
Authors: Shi, Hua-shan, Yang, Li-ping, Wei, Wei, Su, Xiao-qin, Li, Xiao-pen, Li, Meng, Luo, Shun-tao, Zhang, Hai-long, Lu, Lian, Mao, Yong-qiu, Kan, Bing, Yang, Li
J Transl Med, 2013;11(0):86.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot