Human SF20/MYDGF Alexa Fluor® 647-conjugated Antibody

Catalog #: AF1147R Datasheet / COA / SDS

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AF1147R-100UG

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All SF20/MYDGF Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SF20/MYDGF Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human SF20/MYDGF in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse SF20/MYDGF is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SF20/MYDGF
Val32-Leu173
Accession # Q969H8

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SF20/MYDGF

Human SF20 (Stromal cell-derived growth factor SF20, C19orf10, MYDGF1, Myeloid-derived growth factor1 also formerly known as IL25) is a 15 kDa (predicted) bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction. SF20 stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1 mediated signaling pathway. It is thought to inhibit cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway and is involved in endothelial cell proliferation and angiogenesis. Human SF20 shares 87% aa identity with mouse SF20.

Long Name

Stromal Cell-derived Growth Factor SF20

Entrez Gene IDs

56005 (Human); 28106 (Mouse)

Alternate Names

C19orf10; chromosome 19 open reading frame 10; EUROIMAGE1875335; IL25; IL27; IL27w; interleukin 27 working designation; interleukin-25; Ly6elg; MYDGF; myeloid-derived growth factor; R33729_1; SF20; Stromal cell-derived growth factor SF20; UPF0556 protein C19orf10