Human sFRP‑4 Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # AF1827AFP680

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Polyclonal Goat IgG
View all sFRP-4 Primary Antibodies »

Product Specifications

Specificity

Detects human sFRP-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) sFRP-4 is observed, and less than 5% cross-reactivity with recombinant human (rh) sFRP-3, rhsFRP-1, and rmsFRP-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human sFRP‑4 Alexa Fluor™ Plus 680‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: sFRP-4

sFRP-4, also known as DDC-4, Frp, FRPHE and Secreted Frizzled Related Protein 4, is a 50-55 kDa glycoprotein expressed in brain, kidney, lung, ovary, prostate, mammary gland, and endometrium (1, 2). This protein shows complex functions with respect to cell survival: it is up‑regulated with apoptosis during ovulation (3), regulates apoptosis in chondrocytes (4), and promotes apoptosis in mammary glands when expressed in transgenic mice (5). On the other hand, sFRP-4 can also act to enhance growth as it is up‑regulated in endometrial and breast carcinomas (6, 7). Since it is not detected in other carcinomas such as the ovary, colon, and pancreas, this suggest that its role in cancer is likely to be tissue dependent (6). In addition sFRP-4 is characterized as a circulating phosphaturic factor expressed by tumors associated with osteomalacia that antagonizes renal Wnt signaling (7). Of all the secreted frizzled related proteins, sFRP-4 is most closely related to sFRP-3 (1). Mouse and human sFRP-4 proteins share 92% aa identity.

References

  1. Jones, S.E. and C. Jomary (2002) Bioessays 24:811.
  2. Wolf, V. et al. (1997) FEBS Letters 417:385.
  3. Drake, J.M. et al. (2003) Apoptosis 8:389.
  4. James, I.E. et al. (2000) Osteoarthritis & Cartilage 8:452.
  5. Lacher, M.D. et al. (2003) Cell Death Differ. 10:528.
  6. Abu-Jawdeh, G. et al. (1999) Lab Investigation 79:439.
  7. Wong, S.C.C. et al. (2002) J. Pathology 196:145.
  8. Berndt, T. et al. (2003) J. Clin. Invest. 112:785.

Long Name

Secreted Frizzled Related Protein 4

Alternate Names

DDC-4, FRP-AP, frpHE, FrzB2, sFRP4

Entrez Gene IDs

6424 (Human); 20379 (Mouse); 89803 (Rat)

Gene Symbol

SFRP4

UniProt

AAC04617

Additional sFRP-4 Products

Product Documents for Human sFRP‑4 Alexa Fluor™ Plus 680‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human sFRP‑4 Alexa Fluor™ Plus 680‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Wnt Family
Wnt Inhibitors

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