Detects human sFRP-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) sFRP-4 is observed, and less than 5% cross-reactivity with recombinant human (rh) sFRP-3, rhsFRP-1, and rmsFRP-2 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human sFRP-4 Ala22-Val346 Accession # AAC04617
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human sFRP‑4 by Western Blot. Western blot shows lysates of OVCAR‑3 human ovarian carcinoma cell line, human ovarian cancer tissue, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human sFRP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1827) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for sFRP‑4 at approximately 53-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
sFRP-4, also known as DDC-4, Frp, FRPHE and Secreted Frizzled Related Protein 4, is a 50-55 kDa glycoprotein expressed in brain, kidney, lung, ovary, prostate, mammary gland, and endometrium (1, 2). This protein shows complex functions with respect to cell survival: it is up‑regulated with apoptosis during ovulation (3), regulates apoptosis in chondrocytes (4), and promotes apoptosis in mammary glands when expressed in transgenic mice (5). On the other hand, sFRP-4 can also act to enhance growth as it is up‑regulated in endometrial and breast carcinomas (6, 7). Since it is not detected in other carcinomas such as the ovary, colon, and pancreas, this suggest that its role in cancer is likely to be tissue dependent (6). In addition sFRP-4 is characterized as a circulating phosphaturic factor expressed by tumors associated with osteomalacia that antagonizes renal Wnt signaling (7). Of all the secreted frizzled related proteins, sFRP-4 is most closely related to sFRP-3 (1). Mouse and human sFRP-4 proteins share 92% aa identity.
Jones, S.E. and C. Jomary (2002) Bioessays 24:811.
Wolf, V. et al. (1997) FEBS Letters 417:385.
Drake, J.M. et al. (2003) Apoptosis 8:389.
James, I.E. et al. (2000) Osteoarthritis & Cartilage 8:452.
Lacher, M.D. et al. (2003) Cell Death Differ. 10:528.
Abu-Jawdeh, G. et al. (1999) Lab Investigation 79:439.
Wong, S.C.C. et al. (2002) J. Pathology 196:145.
Berndt, T. et al. (2003) J. Clin. Invest. 112:785.
Secreted Frizzled Related Protein 4
Entrez Gene IDs:
DDC-4; Frizzled protein, human endometrium; FRP-4; FRP-AP; frpHE; FRPHEMGC26498; FrzB2; secreted frizzled-related protein 4; secreted frizzled-related protein 4; secreted frizzled-related protein 4; sFRP4; sFRP-4
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