Key Product Details

Species Reactivity

Validated:

Human

Cited:

Rat

Applications

Validated:

Western Blot

Cited:

ELISA Detection

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all sFRP-4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human sFRP-4
Ala22-Val346
Accession # AAC04617

Specificity

Detects human sFRP-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) sFRP-4 is observed, and less than 5% cross-reactivity with recombinant human (rh) sFRP-3, rhsFRP-1, and rmsFRP-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human sFRP‑4 Antibody

Detection of Human sFRP-4 antibody by Western Blot.

Detection of Human sFRP‑4 by Western Blot.

Western blot shows lysates of OVCAR-3 human ovarian carcinoma cell line, human ovarian cancer tissue, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human sFRP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1827) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for sFRP-4 at approximately 53-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human sFRP‑4 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: OVCAR‑3 human ovarian carcinoma cell line, human ovarian cancer tissue, and MCF‑7 human breast cancer cell line

Reviewed Applications

Read 3 reviews rated 4 using AF1827 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: sFRP-4

sFRP-4, also known as DDC-4, Frp, FRPHE and Secreted Frizzled Related Protein 4, is a 50-55 kDa glycoprotein expressed in brain, kidney, lung, ovary, prostate, mammary gland, and endometrium (1, 2). This protein shows complex functions with respect to cell survival: it is up‑regulated with apoptosis during ovulation (3), regulates apoptosis in chondrocytes (4), and promotes apoptosis in mammary glands when expressed in transgenic mice (5). On the other hand, sFRP-4 can also act to enhance growth as it is up‑regulated in endometrial and breast carcinomas (6, 7). Since it is not detected in other carcinomas such as the ovary, colon, and pancreas, this suggest that its role in cancer is likely to be tissue dependent (6). In addition sFRP-4 is characterized as a circulating phosphaturic factor expressed by tumors associated with osteomalacia that antagonizes renal Wnt signaling (7). Of all the secreted frizzled related proteins, sFRP-4 is most closely related to sFRP-3 (1). Mouse and human sFRP-4 proteins share 92% aa identity.

References

  1. Jones, S.E. and C. Jomary (2002) Bioessays 24:811.
  2. Wolf, V. et al. (1997) FEBS Letters 417:385.
  3. Drake, J.M. et al. (2003) Apoptosis 8:389.
  4. James, I.E. et al. (2000) Osteoarthritis & Cartilage 8:452.
  5. Lacher, M.D. et al. (2003) Cell Death Differ. 10:528.
  6. Abu-Jawdeh, G. et al. (1999) Lab Investigation 79:439.
  7. Wong, S.C.C. et al. (2002) J. Pathology 196:145.
  8. Berndt, T. et al. (2003) J. Clin. Invest. 112:785.

Long Name

Secreted Frizzled Related Protein 4

Alternate Names

DDC-4, FRP-AP, frpHE, FrzB2, sFRP4

Entrez Gene IDs

6424 (Human); 20379 (Mouse); 89803 (Rat)

Gene Symbol

SFRP4

UniProt

AAC04617

Additional sFRP-4 Products

Product Documents for Human sFRP‑4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human sFRP‑4 Antibody

For research use only

Related Research Areas

Wnt Family
Wnt Inhibitors

Citations for Human sFRP‑4 Antibody

Customer Reviews for Human sFRP‑4 Antibody (3)

4 out of 5
3 Customer Ratings
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4 Stars
100%
3 Stars
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1 Stars
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Showing  1 - 3 of 3 reviews Showing All
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  • Human sFRP-4 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human sFRP‑4 Antibody AF1827
  • Human sFRP-4 Antibody
    Name: Ed Lo
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human and Mouse
    Verified Customer | Posted 11/07/2018
  • Human sFRP-4 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 10/31/2018

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