Human Siglec-11 Alexa Fluor® 350-conjugated Antibody

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FAB3258U-100UG
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Human Siglec-11 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Siglec-11 in direct ELISAs. In direct ELISAs, no cross-reactivity with Siglec-16 is observed.
Source
Monoclonal Mouse IgG2a Clone # 705904
Immunogen
Human Siglec-11 synthetic peptide
PPARLSWTRWGQTVGPSQPSDPGVLELPPIC
Accession # Q96RL6
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 350

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
HEK293 human embryonic kidney cell line transfected with human Siglec-11

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Siglec-11

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N‑terminal Ig-like V-set domain which mediates sialic acid binding, followed by a varying numbers of Ig-like C2-set domains. Siglecs‑3 and 5‑13 constitute the CD33/Siglec-3 related group, which are defined by their sequence homology and differential expression in the hematopoietic system (1‑3). Mature human Siglec-11 consists of a 534 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 114 aa cytoplasmic domain. The ECD contains one Ig-like V-set domain, and three Ig-like C2-set domains. The cytoplasmic domain contains two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (4). A splice variant of Siglec-11 has a deletion of nearly 100 aa in the extracellular juxtamembrane region. Among siglecs, the ECD of Siglec-11 is most closely related to that of Siglec-10 (82% aa sequence identity). The cytoplasmic domains of these proteins are only 20% identical. Siglec-11 is closely related to the pseudogenes Siglec-14 and Siglec-16 (4, 5). Human Siglec-11 shares 90%‑96% aa sequence identity with Siglec-11 from great apes. Rodent orthologs of Siglec-11 have not been identified. In human, Siglec-11 is expressed in tissue macrophages, brain microglia, and inflammatory site monocytes (4). Strong microglial expression is specific to humans, as it is less prominent or absent in chimpanzees and orangutans (5). Siglec-11 forms 180 kDa disulfide-linked dimers. It shows a strong binding preference for sialic acid in alpha 2-8 linkage which is unusual for siglecs (4). A conserved arginine in the Ig-like V-set domain only partially contributes to Siglec-11 ligand recognition, in contrast to its being required in other siglecs (4). Tyrosine phosphorylation of the cytoplasmic region of Siglec-11 promotes association with the phosphatases SHP-1 and SHP-2 (4).

References
  1. Varki, A. and T. Angata (2006) Glycobiology 16:1R.
  2. Crocker, P.R. (2005) Curr. Opin. Pharmacol. 5:431.
  3. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
  4. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
  5. Hayakawa, T. et al. (2005) Science 309:1693.
Long Name
Sialic Acid Binding Ig-like Lectin 11
Entrez Gene IDs
114132 (Human)
Alternate Names
sialic acid binding Ig-like lectin 11; sialic acid-binding Ig-like lectin 11; Sialic acid-binding lectin 11; Siglec11; Siglec-11

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