Human Smad3 Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
IC4038T-100UG
Product Details
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Human Smad3 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Smad3 in direct ELISAs.
Source
Monoclonal Rat IgG1 Clone # 378611
Immunogen
E. coli-derived recombinant human Smad3
Ser2-Ala230
Accession # P84022
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
PC‑3 human prostate cancer cell line fixed with paraformaldehyde and permeabilized with methanol

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Smad3

Smad3 is phosphorylated in cells stimulated with TGF-beta, complexes with Smad4, and translocates to the nucleus to upregulate gene transcription. Smad3 is critical for signaling fibrosis and wound healing.

Long Name
Mothers Against DPP Homolog 3
Entrez Gene IDs
4088 (Human)
Alternate Names
DKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; HsT17436; JV15-2MGC60396; MAD homolog 3; mad protein homolog; MAD, mothers against decapentaplegic homolog 3 (Drosophila); MAD, mothers against decapentaplegic homolog 3; mad3; MADH3mad homolog JV15-2; mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD 3; SMAD family member 3HSPC193; SMAD, mothers against DPP homolog 3 (Drosophila); SMAD, mothers against DPP homolog 3; Smad3

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