Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 378611
View all Smad3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Smad3
Ser2-Ala230
Accession # P84022

Specificity

Detects human Smad3 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Human Smad3 Antibody

Smad3 antibody in Human Pancreatic Cancer Tissue by Immunohistochemistry (IHC-P).

Smad3 in Human Pancreatic Cancer Tissue.

Smad3 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using 15 µg/mL Rat Anti-Human Smad3 Monoclonal Antibody (Catalog # MAB4038) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Smad3 antibody in PC‑3 Human Cell Line antibody by Flow Cytometry.

Detection of Smad3 in PC‑3 Human Cell Line by Flow Cytometry.

PC‑3 human prostate cancer cell line was stained with Rat Anti-Human Smad3 Monoclonal Antibody (Catalog # MAB4038, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
Smad3 antibody in MDA-MB-231 Human Cell Line by Immunocytochemistry (ICC).

Smad3 in MDA‑MB‑231 Human Cell Line.

Smad3 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Rat Anti-Human Smad3 Monoclonal Antibody (Catalog # MAB4038) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Smad3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: PC‑3 human prostate cancer cell line fixed with paraformaldehyde and permeabilized with methanol

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Smad3

Smad3 is phosphorylated in cells stimulated with TGF-beta, complexes with Smad4, and translocates to the nucleus to upregulate gene transcription. Smad3 is critical for signaling fibrosis and wound healing.

Long Name

Mothers Against DPP Homolog 3

Alternate Names

DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3

Entrez Gene IDs

4088 (Human)

Gene Symbol

SMAD3

UniProt

P84022

Additional Smad3 Products

Product Documents for Human Smad3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Smad3 Antibody

For research use only

Citations for Human Smad3 Antibody

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Protocols

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FAQs

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